| Autor: |
van Hattum, J. W., Scutigliani, E. M., Helderman, R. F. C. P. A., Zweije, R., Rodermond, H. M., Oei, A. L., Crezee, J., Oddens, J. R., de Reijke, T. M., Krawczyk, P. M. |
| Přispěvatelé: |
Graduate School, Medical Biology, ANS - Cellular & Molecular Mechanisms, ANS - Neurodegeneration, CCA - Cancer biology and immunology, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Center of Experimental and Molecular Medicine, Radiotherapy, Urology, APH - Personalized Medicine, APH - Quality of Care |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Scientific reports, 12(1):7017. Nature Publishing Group |
| ISSN: |
2045-2322 |
| DOI: |
10.1038/s41598-022-11016-y |
| Popis: |
Hyperthermic intravesical chemotherapy (HIVEC)—whereby the bladder is heated to ± 43 °C during a chemotherapy instillation—can improve outcomes of non-muscle invasive bladder cancer (NMIBC) treatments. Experiments in animal models are required to explore new hyperthermia based treatments. Existing HIVEC devices are not suitable for rodents or large-scale animal trials. We present a HIVEC setup compatible with orthotopic rat models. An externally heated chemotherapeutic solution is circulated in the bladder through a double-lumen catheter with flow rates controlled using a peristaltic pump. Temperature sensors in the inflow channel, bladder and outflow channel allow temperature monitoring and adjustments in real-time. At a constant flow rate of 2.5 mL/min the system rapidly reaches the desired bladder temperature of 42–43 °C with minimal variability throughout a one-hour treatment in a rat bladder phantom, as well as in euthanised and live rats. Mean intraluminal bladder temperatures were 42.92 °C (SD = 0.15 °C), 42.45 °C (SD = 0.37 °C) and 42.52 °C (SD = 0.09 °C) in the bladder phantom, euthanised, and live rats respectively. Thermal camera measurements showed homogenous heat distributions over the bladder wall. The setup provides well-controlled thermal dose and the upscaling needed for performing large scale HIVEC experiments in rats. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full text from SpringerLink