BapC autotransporter protein is a virulence determinant of Bordetella pertussis
| Autor: | Roger Parton, John G. Coote, Mark Roberts, Paul Blackburn, Habib Bokhari, Mojtaba Noofeli |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Serum Blood Bactericidal Activity Bordetella pertussis Whooping Cough Pseudogene Molecular Sequence Data Mutant Virulence Microbiology Frameshift mutation Rodent Diseases Mice Animals Humans Amino Acid Sequence Virulence Factors Bordetella Gene Genetics Microbial Viability Polymorphism Genetic Base Sequence biology Sequence Analysis DNA biology.organism_classification Disease Models Animal Infectious Diseases GenBank Autotransporter domain Female Sequence Alignment Gene Deletion Bacterial Outer Membrane Proteins |
| Zdroj: | Microbial Pathogenesis. 51:169-177 |
| ISSN: | 0882-4010 |
| Popis: | A protein designated Bap-5 (GenBank accession no. AF081494 ) or BapC (GenBank accession no. AJ277634 ) has been identified as a member of the Bordetella pertussis autotransporter family and the present work suggests that this protein, like the previously characterised BrkA, is a Bvg-regulated serum resistance factor and virulence determinant. B. pertussis bapC and brkA , bapC mutants were created and, like a brkA mutant, showed greater sensitivity to killing by normal human serum than their parent strains but they were not as sensitive as a bvg mutant. Competition assays also showed an important role for BapC, like BrkA, in virulence of B. pertussis in mice after intranasal infection. Moreover, the bapC and brkA , bapC mutants, like the brkA mutant, were found to be more sensitive to the antimicrobial peptide cecropin P1 than the parent strains. In the genome sequence of B. pertussis strain Tohama, bapC is designated as a pseudogene due, in part, to a frameshift in a poly(C) tract near the 5′ end of the gene which creates a truncated BapC protein. Sequence analyses of the bapC region spanning the poly(C) tract of a number of B. pertussis strains showed minor nucleotide and amino acid polymorphisms but it appeared that all had an ORF that would be able to produce BapC. |
| Databáze: | OpenAIRE |
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