Identification of Commensal Species Positively Correlated with Early Stress Responses to a Compromised Mucus Barrier
| Autor: | Bruno Sovran, Linda M. P. Loonen, Floor Hugenholtz, Paul de Vos, Michiel Kleerebezem, Jerry M. Wells, Clara Belzer, Ellen H. Stolte, Hauke Smidt, Peter van Baarlen, Mark V. Boekschoten, Peng Lu, Jan P. Dekker, Ingrid B. Renes |
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| Přispěvatelé: | Man, Biomaterials and Microbes (MBM), Translational Immunology Groningen (TRIGR), Pediatrics, Academic Medical Center |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
HOST colitis Inflammatory bowel disease Voeding Metabolisme en Genomica Mice Microbiologie Immunology and Allergy stress markers Directie Intestinal Mucosa Oligonucleotide Array Sequence Analysis Mice Knockout Microbiota Gastroenterology respiratory system Colitis Ulcerative colitis Metabolism and Genomics CROHNS-DISEASE ULCERATIVE-COLITIS ESCHERICHIA-COLI Metabolisme en Genomica TRANSGENIC RATS BACTERIA Nutrition Metabolism and Genomics Female MUCIN MUC2 Biology Microbiology digestive system 03 medical and health sciences Immune system Voeding Muc2 deficiency Stress Physiological medicine Animals Humans Host-Microbe Interactomics Nutrition VLAG Mucin-2 Bacteroidetes BACTEROIDES-VULGATUS Gene Expression Profiling Wild type Heterozygote advantage medicine.disease biology.organism_classification Mucus digestive system diseases 030104 developmental biology Gene Expression Regulation Immunology WIAS Bacteroides INFLAMMATORY-BOWEL-DISEASE |
| Zdroj: | Inflammatory Bowel Diseases, 22(4), 826-840. LIPPINCOTT WILLIAMS & WILKINS Inflammatory Bowel Diseases, 22(4), 826-840 Inflammatory Bowel Diseases, 22(4), 826-840. Oxford University Press Inflammatory bowel diseases, 22(4), 826-840. John Wiley and Sons Inc. Inflammatory Bowel Diseases 22 (2016) 4 |
| ISSN: | 1078-0998 |
| DOI: | 10.1097/mib.0000000000000688 |
| Popis: | Background: Our aims were (1) to correlate changes in the microbiota to intestinal gene expression before and during the development of colitis in Muc2(-/-) mice and (2) to investigate whether the heterozygote Muc2(+/-) mouse would reveal host markers of gut barrier stress. Methods: Colon histology, transcriptomics, and microbiota profiling of faecal samples was performed on wild type, Muc2(+/-), and Muc2(-/-) mice at 2, 4, and 8 weeks of age. Results: Muc2(-/-) mice develop colitis in proximal colon after weaning, resulting in inflammatory and adaptive immune responses, and expression of genes associated with human inflammatory bowel disease. Muc2(+/-) mice do not develop colitis, but produce a thinner mucus layer. The transcriptome of Muc2(+/-) mice revealed differential expression of genes participating in mucosal stress responses and exacerbation of a transient inflammatory state around the time of weaning. Young wild type and Muc2(+/-) mice have a more constrained group of bacteria as compared with the Muc2(-/-) mice, but at 8 weeks the microbiota composition is more similar in all mice. At all ages, microbiota composition discriminated the groups of mice according to their genotype. Specific bacterial clusters correlated with altered gene expression responses to stress and bacteria, before colitis development, including colitogenic members of the genus Bacteroides. Conclusions: The abundance of Bacteroides pathobionts increased before histological signs of pathology suggesting they may play a role in triggering the development of colitis. The Muc2(+/-) mouse produces a thinner mucus layer and can be used to study mucus barrier stress in the absence of colitis. |
| Databáze: | OpenAIRE |
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