Virus replication and virion export in X-deficient hepatitis B virus transgenic mice
| Autor: | Jürgen Löhler, Fritz von Weizsäcker, Gabriele Spindler, Christa Kuhn, Josef Köck, Petra Nusser, Kurt Reifenberg |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Hepatitis B virus
Hepatitis B virus DNA polymerase viruses Transgene Mice Transgenic Biology Virus Replication medicine.disease_cause Hepatitis B virus PRE beta Gene product Mice chemistry.chemical_compound Virology medicine Animals Viral Regulatory and Accessory Proteins Virion Molecular biology digestive system diseases Mice Inbred C57BL HBx Viral replication chemistry Mice Inbred DBA Trans-Activators DNA |
| Zdroj: | Journal of General Virology. 83:991-996 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | The function of the X protein (pX) in the replication cycle of mammalian hepadnaviruses is enigmatic. Using tissue culture experiments it has been shown that the X gene product is not central to hepatitis B virus (HBV) replication and virion export. However, at present it is still unclear whether this also applies to the in vivo situation. Using a terminally redundant X-deficient HBV DNA construct, transgenic mice were established that exhibited high-level expression of the viral core protein in liver and kidneys. Importantly, replicative DNA intermediates and mature viral genomes could be detected in the liver and serum of these mice, respectively. These findings indicate that, in the in vivo model of transgenic mice, the HBV X (HBx) gene product is not required for HBV replication and virion secretion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|