Development of the Large-Scale Oligonucleotide Chip for the Diagnosis of Plant Viruses and its Practical Use
| Autor: | Seung Mo Lim, Jae Sun Moon, Jeong Seon Kim, Chung Youl Park, Hyoun-Sub Lim, Moon Nam, Hong-Soo Choi, Jeong Gyu Kim, Su Heon Lee |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Genetics
LSON chip education.field_of_study Oligonucleotide Large-scale oligonucleotide chip viruses Population Nucleic acid sequence Satellite RNAs oligo chip Biology lcsh:Plant culture Oligonucleotide chip Genome Virus plant virus virus diagnosis Plant virus lcsh:SB1-1110 education Agronomy and Crop Science Research Article |
| Zdroj: | The Plant Pathology Journal, Vol 30, Iss 1, Pp 51-57 (2014) The Plant Pathology Journal |
| ISSN: | 1598-2254 |
| Popis: | A large-scale oligonucleotide (LSON) chip was developed for the detection of the plant viruses with known genetic information. The LSON chip contains two sets of 3,978 probes for 538 species of targets including plant viruses, satellite RNAs and viroids. A hundred forty thousand probes, consisting of isolate-, species- and genus-specific probes respectively, are designed from 20,000 of independent nucleotide sequence of plant viruses. Based on the economic importance, the amount of genome information, and the number of strains and/or isolates, one to fifty-one probes for each target virus are selected and spotted on the chip. The standard and field samples for the analysis of the LSON chip have been prepared and tested by RT-PCR. The probe's specific and/or nonspecific reaction patterns by LSON chip allow us to diagnose the unidentified viruses. Thus, the LSON chip in this study could be highly useful for the detection of unexpected plant viruses, the monitoring of emerging viruses and the fluctuation of the population of major viruses in each plant. |
| Databáze: | OpenAIRE |
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