Single-cell transcriptome analysis of avian neural crest migration reveals signatures of invasion and molecular transitions
Autor: | Andrew C. Box, Hua Li, Craig L. Semerad, Mary Cathleen McKinney, Kathryn E Hall, Madelaine Gogol, Jason A. Morrison, Samuel Meier, Anoja Perera, Rebecca McLennan, Jessica M. Teddy, Lauren A. Wolfe, Paul M. Kulesa, Laura Holmes |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
QH301-705.5 Science Cell Chick Embryo Biology migration trailblazers General Biochemistry Genetics and Molecular Biology Transcriptome 03 medical and health sciences Spatio-Temporal Analysis Cranial neural crest Cell Movement medicine Animals Single cell Biology (General) 10. No inequality Gene knockdown General Immunology and Microbiology Gene Expression Profiling General Neuroscience Neural crest Embryo Cell Biology General Medicine RNAseq Chicken Tools and Resources Cell biology Developmental Biology and Stem Cells 030104 developmental biology medicine.anatomical_structure Medicine Single-Cell Analysis Stem cell Developmental biology neural crest |
Zdroj: | eLife, Vol 6 (2017) eLife |
Popis: | Neural crest cells migrate throughout the embryo, but how cells move in a directed and collective manner has remained unclear. Here, we perform the first single-cell transcriptome analysis of cranial neural crest cell migration at three progressive stages in chick and identify and establish hierarchical relationships between cell position and time-specific transcriptional signatures. We determine a novel transcriptional signature of the most invasive neural crest Trailblazer cells that is consistent during migration and enriched for approximately 900 genes. Knockdown of several Trailblazer genes shows significant but modest changes to total distance migrated. However, in vivo expression analysis by RNAscope and immunohistochemistry reveals some salt and pepper patterns that include strong individual Trailblazer gene expression in cells within other subregions of the migratory stream. These data provide new insights into the molecular diversity and dynamics within a neural crest cell migratory stream that underlie complex directed and collective cell behaviors. |
Databáze: | OpenAIRE |
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