A simple approach for mouse embryonic stem cells isolation and differentiation inducing embryoid body formation
| Autor: | Pablo Argibay, Carol Betiana Fagundez, Marcos Ojea Quintana, Sebastian Gogorza, Monica Loresi, Stella M Delcourt, Roberto Testa |
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| Rok vydání: | 2009 |
| Předmět: |
KOSR
Cellular differentiation Cell Culture Techniques Lewis X Antigen Cell Separation Embryoid body Immunosurgery Biology Morula Mice Animals Embryonic Stem Cells Cell Differentiation Cell Biology General Medicine Molecular biology Embryonic stem cell Culture Media Cell biology Mice Inbred C57BL Blastocyst Cell culture embryonic structures Alkaline phosphatase Stem cell Octamer Transcription Factor-3 |
| Zdroj: | Cell Biology International. 33:1196-1200 |
| ISSN: | 1065-6995 |
| Popis: | Stem cells were derived from hatched blastocyst-stage mouse embryos of the C57BL/6 strain employing a knockout serum replacement instead of the traditional fetal calf serum, thereby avoiding the use of immunosurgery. Although fetal calf serum was not good for isolation of stem cells, a combination of this serum plus knockout serum increased the expansion rate of the cell culture. The derived cells were capable of maintaining an undifferentiated state during several passages, as demonstrated by the presence of alkaline phosphatase activity, stage-specific embryonic antigen 1 (SSEA-1), and octamer binding protein 4 (Oct-4). Suspension culture in bacteriological dishes gave better results than the hanging drop method for differentiation by means of embryoid body formation. Mouse embryonic stem cells showed spontaneous differentiation into derivatives of the 3 germ layers in culture media supplemented with fetal calf serum but not with knockout serum. |
| Databáze: | OpenAIRE |
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