Detection of protein–protein interactions on SiO2/Si surfaces by spectroscopic ellipsometry
| Autor: | Hiroshi Iwasaki, Tatsuo Yoshinobu, Masamitsu Futai, Toshihide Okajima, Katsuyuki Tanizawa, Daisuke Kitaguchi, Hidehiko Iwabuki, Seigo Kodera, Shun'ichi Kuroda |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Silicon
Infrared Rays Infrared Recombinant Fusion Proteins Biophysics Plasma protein binding Ligands Biochemistry Antibodies Protein–protein interaction Hemoglobins Mice chemistry.chemical_compound Antigen Antibody Specificity Animals Humans Antigens Biochip Molecular Biology Epidermal Growth Factor Myoglobin Chemistry Ligand Spectrum Analysis Proteins Cell Biology Silicon Dioxide ErbB Receptors Crystallography Monomer Rabbits Bacteriophage M13 Protein Binding |
| Zdroj: | Analytical Biochemistry. 321:65-70 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/s0003-2697(03)00422-6 |
| Popis: | We have applied spectroscopic ellipsometry to sensitive detection of specific protein-protein interactions on SiO2/Si substrates. First, the change of ellipticity of the reflected polarized light (600-1100 nm) was correlated with the thickness of the protein layer immobilized on SiO2/Si surfaces by measuring monomeric (myoglobin) and homotetrameric (hemoglobin) proteins with a similar monomer size. Protein-protein interactions were then measured with the antigen/antibody and cell-surface receptor/ligand systems; in each system either of the two proteins was bound to SiO2/Si substrates. Consequently, significant ellipticity changes were observed only for the cases where the interactions were specific. A specific antibody binding was also detectable with an antigen displayed on the surface of bacteriophage particles. These results show the usefulness of spectroscopic ellipsometry for sensitive detection of protein-protein interactions and its applicability to a detection method for the protein-based biochips to be developed in the future. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect