Decolorization and biodegradation of Reactive Blue 13 by Proteus mirabilis LAG
| Autor: | Akinniyi Adediran Osuntoki, George O. Gbenle, O.D. Olukanni, Dayanand Kalyani, Sanjay P. Govindwar |
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| Rok vydání: | 2010 |
| Předmět: |
Environmental Engineering
Tropylium cation Health Toxicology and Mutagenesis Color Mineralization (biology) Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Spectrophotometry medicine Environmental Chemistry Coloring Agents Proteus mirabilis Waste Management and Disposal Naphthalene chemistry.chemical_classification Laccase Chromatography medicine.diagnostic_test biology Aromatic amine Biodegradation biology.organism_classification Pollution Biodegradation Environmental chemistry Nuclear chemistry |
| Zdroj: | Journal of Hazardous Materials. 184:290-298 |
| ISSN: | 0304-3894 |
| DOI: | 10.1016/j.jhazmat.2010.08.035 |
| Popis: | The decolorization and biodegradation of Reactive Blue 13 (RB13), a sulphonated reactive azo dye, was achieved under static anoxic condition with a bacterial strain identified as Proteus mirabilis LAG, which was isolated from a municipal dump site soil near Lagos, Nigeria. This strain decolorized RB13 (100 mg/l) within 5 h. The formation of aromatic amine prior to mineralization was supported by Fourier transform infrared spectrometry (FTIR), which revealed the disappearance of certain peaks, particularly those of the aromatic C–H bending at 600–800 cm−1. Gas chromatography–mass spectrophotometry (GCMS) analysis of the dye metabolite showed the presence of sodium-2(2-formyl-2-hydroxyvinyl) benzoate, with a tropylium cation as its base peak, this suggested the breakage of naphthalene rings in RB13. The detection of azoreductase and laccase activities suggested the enzymatic reduction of azo bonds prior to mineralization. In addition, phytotoxicity studies indicated the detoxification of RB13 to non-toxic degradation products by this strain of P. mirabilis LAG. |
| Databáze: | OpenAIRE |
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