NIR Fluorescent Imaging and Photodynamic Therapy with a Novel Theranostic Phospholipid Probe for Triple-Negative Breast Cancer Cells
| Autor: | Theresa M. Busch, Alejandro D. Arroyo, Benjamin K. Liebov, Natalia I. Rubtsova, Michael C Hart, Nadia Anikeeva, Anatoliy V. Popov, Sanghoon Chong, Joann Miller, E. James Delikatny, Sofya A Osharovich, Jeffrey M. Cochran, Min Yuan, Arjun G. Yodh |
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| Rok vydání: | 2021 |
| Předmět: |
Fluorophore
Spectrophotometry Infrared Cell Survival medicine.medical_treatment Biomedical Engineering Pharmaceutical Science Triple Negative Breast Neoplasms Bioengineering Photodynamic therapy 02 engineering and technology 01 natural sciences chemistry.chemical_compound Prostate Cell Line Tumor medicine Humans Phospholipids Triple-negative breast cancer Cell Proliferation Pharmacology Molecular Structure 010405 organic chemistry Organic Chemistry Photosensitizing Agent 021001 nanoscience & nanotechnology Lipids Fluorescence In vitro 0104 chemical sciences medicine.anatomical_structure Photochemotherapy chemistry Drug Design Cancer cell Cancer research Nanoparticles 0210 nano-technology Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 32:1852-1863 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | New exogenous probes are needed for both imaging diagnostics and therapeutics. Here, we introduce a novel nanocomposite near-infrared (NIR) fluorescent imaging probe and test its potency as a photosensitizing agent for photodynamic therapy (PDT) against triple-negative breast cancer cells. The active component in the nanocomposite is a small molecule, pyropheophorbide a-phosphatidylethanolamine-QSY21 (Pyro-PtdEtn-QSY), which is imbedded into lipid nanoparticles for transport in the body. The probe targets abnormal choline metabolism in cancer cells; specifically, the overexpression of phosphatidylcholine-specific phospholipase C (PC-PLC) in breast, prostate, and ovarian cancers. Pyro-PtdEtn-QSY consists of a NIR fluorophore and a quencher, attached to a PtdEtn moiety. It is selectively activated by PC-PLC resulting in enhanced fluorescence in cancer cells compared to normal cells. In our in vitro investigation, four breast cancer cell lines showed higher probe activation levels than noncancerous control cells, immortalized human mammary gland cells, and normal human T cells. Moreover, the ability of this nanocomposite to function as a sensitizer in PDT experiments on MDA-MB-231 cells suggests that the probe is promising as a theranostic agent. |
| Databáze: | OpenAIRE |
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