Prolonging the shelf life ofLumbricus terrestriserythrocruorin for use as a novel blood substitute
| Autor: | Osheiza Abdulmalik, Jacob Elmer, Christopher Neely, Christine Muzzelo, Payal Shah |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors Antioxidant Drug Storage medicine.medical_treatment Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Heme 030204 cardiovascular system & hematology Antioxidants Blood substitute Ferrous Hemoglobins 03 medical and health sciences 0302 clinical medicine Drug Stability Blood Substitutes medicine Animals Erythrocruorin Food science Oligochaeta Deoxygenation biology Chemistry Temperature General Medicine Ascorbic acid biology.organism_classification Oxygen 030104 developmental biology Biochemistry biology.protein Hemoglobin Oxidation-Reduction Lumbricus terrestris Biotechnology |
| Zdroj: | Artificial Cells, Nanomedicine, and Biotechnology. 46:39-46 |
| ISSN: | 2169-141X 2169-1401 |
| Popis: | Limitations associated with the storage of red blood cells have motivated the development of novel blood substitutes that are able to withstand long-term storage at elevated temperatures. The hemoglobin of the earthworm Lumbricus terrestris (LtEc) is an attractive blood substitute candidate, since it is resistant to oxidation and aggregation during storage. Several factors were investigated to optimize the thermal and oxidative stability of LtEc during storage, including pH, antioxidant supplements, and deoxygenation. A strategy for the reduction of fully oxidized LtEc with antioxidants was also developed. Overall, LtEc was shown to have the highest thermal stability in Ringer’s Modified Lactate solution with 10 mM HEPES at pH 7.0. Deoxygenation of the LtEc was also shown to significantly reduce oxidation of the ferrous heme iron (e.g., %Fe2+ after 7 d at 37 °C = 75.7%). However, even in cases where oxidation does occur, the addition of 1.8 mM ascorbic acid (AA) was found to reduce 98.3% of the ox... |
| Databáze: | OpenAIRE |
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