Nerve Growth Factor Regulates Expression of the Nerve Growth Factor Receptor Gene in Adult Sensory Neurons
| Autor: | T. P. Misko, Georg Dechant, Dan Lindholm, M. J. Radeke, Eric M. Shooter, Hans Thoenen, Ronald M. Lindsay |
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| Rok vydání: | 1990 |
| Předmět: |
Messenger RNA
medicine.medical_specialty biology General Neuroscience Central nervous system Ciliary neurotrophic factor Fibroblast growth factor Sensory neuron medicine.anatomical_structure Nerve growth factor Endocrinology nervous system Dorsal root ganglion Epidermal growth factor Internal medicine medicine biology.protein |
| Zdroj: | European Journal of Neuroscience. 2:389-396 |
| ISSN: | 1460-9568 0953-816X |
| DOI: | 10.1111/j.1460-9568.1990.tb00431.x |
| Popis: | Sensory neurons of the adult rat dorsal root ganglion (DRG) can be maintained in culture in the absence of nerve growth factor (NGF). We have thus used dissociated cultures of these neurons to study effects of NGF on the regulation of expression of mRNA encoding the nerve growth factor receptor (NGF-R). In the absence of NGF, levels of NGF-R mRNA remained constant for 7 days in cultures of adult rat DRG neurons. In the presence of NGF, NGF-R mRNA levels rose two - three-fold after 2 days, reaching plateau levels (five - six-fold elevation) after 5 days. This NGF-induced up-regulation could be demonstrated even after prior NGF-deprivation for 3 - 4 days. NGF had no effect upon NGF-R mRNA levels in DRG non-neuronal cells. Epidermal growth factor (EGF), fibroblast growth factor (FGF) and ciliary neurotrophic factor (CNTF) were without effect on NGF-R mRNA levels, but 8-bromo-cAMP decreased NGF-R mRNA levels by 65% after 2 days. NGF also induced a rapid (30 min) rise in expression of c-fos in DRG neurons, but not in non-neuronal cells. Our results suggest that endogenous levels of NGF may regulate the expression of NGF-R in vivo. |
| Databáze: | OpenAIRE |
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