Morphologic and ultrastructural evidence of interleukin-6 induced platelet activation
| Autor: | E G Puszkin, Janice P. Dutcher, Randi Isaacs, Zbigniew Mrowiec, Leslie Oleksowicz |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Blood Platelets
medicine.medical_treatment Cell Platelet Membrane Glycoproteins Flow cytometry Andrology Adenosine Triphosphate medicine Humans Platelet Platelet activation Interleukin 6 biology medicine.diagnostic_test Chemistry Interleukin-6 Hematology Flow Cytometry Platelet Activation In vitro Recombinant Proteins Microscopy Electron P-Selectin medicine.anatomical_structure Cytokine Immunology biology.protein Microscopy Electron Scanning Dense granule Cell Adhesion Molecules |
| Zdroj: | American journal of hematology. 48(2) |
| ISSN: | 0361-8609 |
| Popis: | The in vitro effect of IL-6 on platelet activation was investigated. When human platelets were incubated with high (1,000 ng/ml) or low (1 ng/ml) dose IL-6, expression of GMP-140 was enhanced by 42% (N = 6; P < 0.009) and 46% (N = 6; P < 0.061) in 1 hr low and high dose IL-6-platelet incubations, respectively, as assessed by flow cytometry. In platelet specimens incubated with high dose IL-6 for 3 hr, a 70% (N = 6; P < 0.009) increase in GMP-140 expression over control was observed. Parallel high dose IL-6 incubations subjected to scanning electron microscopic studies revealed a 3.4-fold increase (N = 6; P < 0.001) in spheroid morphologic platelet forms in 1 hr incubations in comparison to control platelet preparations, whereas in 3 hr IL-6-platelet incubations, a 96% increase in dendritic platelet forms was observed (N = 6; P < 0.001). Significant increases in platelet ATP levels were observed in both 1 min and 1 hr high dose and low dose IL-6 platelet incubations. In 3 hr high dose-IL-6 platelet incubations, a significant 18% (N = 8; P < 0.001) decrease in platelet ATP was parallelled by a significant 40% increase (N = 8; P < 0.014) in plasma ATP in the same specimens. This increased plasma ATP was highly correlated with a reduction in platelet ATP when analyzed by bivariate regression analysis. Lastly, transmission electron microscopic analysis demonstrated a significant reduction in dense granule number and ratio of dense granule surface area/cell surface area in 3 hr high dose IL-6 incubations. These findings suggests that IL-6 activates platelets in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text