Time-resolved fluorescence imaging of islet cell autoantibodies
| Autor: | Olli Simell, Jorma Ilonen, Heikki Hyöty, Tuula Simell, Satu Simell, Maris Rulli, Ari Kuusisto, Pauli Vuorinen, Tero Vahlberg, Mikael Knip |
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| Rok vydání: | 2006 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Bioanalysis Time Factors Serial dilution Lanthanoid Series Elements General Biochemistry Genetics and Molecular Biology 03 medical and health sciences symbols.namesake Islets of Langerhans Nuclear magnetic resonance History and Philosophy of Science Stokes shift Computer software Image Processing Computer-Assisted Humans Child Fluorescent Antibody Technique Indirect 030304 developmental biology Autoantibodies 0303 health sciences Chemistry General Neuroscience 030302 biochemistry & molecular biology Islet cell autoantibodies Fluorescence 3. Good health Diabetes Mellitus Type 1 symbols Time-resolved spectroscopy |
| Zdroj: | Annals of the New York Academy of Sciences. 1079 |
| ISSN: | 0077-8923 |
| Popis: | Time-resolved fluorescence of lanthanide chelates has been widely used in bioanalytical assays. Long fluorescence time, large Stokes shift, and minute fading out of the fluorescence over years are major advantages of the lanthanides over the conventional fluorescent dyes. We have now applied time-resolved fluorescence imaging (TRFI) also for measurement of type 1 diabetes mellitus (T1DM)-related islet cell autoantibodies (ICA). Retaining the accuracy of conventional ICA, TRFI has over 10 times better signal-to-noise ratio than the conventional fluorochromes. The technology allows objective determination of fluorescence intensity with the camera and computer software, and serial dilutions for obtaining the antibody titer in autoantibody-positive samples are unnecessary.We now describe the TRFI as a method and its application for measurement of ICA. |
| Databáze: | OpenAIRE |
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