SOX17 integrates HOXA and arterial programs in hemogenic endothelium to drive definitive lympho-myeloid hematopoiesis
| Autor: | Mi Ae Park, Irene M. Ong, Kran Suknuntha, Gene Uenishi, James A. Thomson, Igor I. Slukvin, Yoon Jung Choi, Ho Sun Jung, Peng Liu, Matthew Raymond |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
animal structures Myeloid Induced Pluripotent Stem Cells Regulator Notch signaling pathway Biology CXCR4 Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine SOXF Transcription Factors medicine Animals Humans Induced pluripotent stem cell Embryonic Stem Cells Homeodomain Proteins Hemogenic endothelium Cell Differentiation Hematopoiesis Cell biology Haematopoiesis 030104 developmental biology medicine.anatomical_structure embryonic structures Stem cell 030217 neurology & neurosurgery |
| Zdroj: | Cell reports |
| ISSN: | 2211-1247 |
| Popis: | SUMMARY SOX17 has been implicated in arterial specification and the maintenance of hematopoietic stem cells (HSCs) in the murine embryo. However, knowledge about molecular pathways and stage-specific effects of SOX17 in humans remains limited. Here, using SOX17-knockout and SOX17-inducible human pluripotent stem cells (hPSCs), paired with molecular profiling studies, we reveal that SOX17 is a master regulator of HOXA and arterial programs in hemogenic endothelium (HE) and is required for the specification of HE with robust lympho-myeloid potential and DLL4+CXCR4+ phenotype resembling arterial HE at the sites of HSC emergence. Along with the activation of NOTCH signaling, SOX17 directly activates CDX2 expression, leading to the upregulation of the HOXA cluster genes. Since deficiencies in HOXA and NOTCH signaling contribute to the impaired in vivo engraftment of hPSC-derived hematopoietic cells, the identification of SOX17 as a key regulator linking arterial and HOXA programs in HE may help to program HSC fate from hPSCs. Graphical Abstract In Brief Jung et al. report that SOX17 is a critical upstream factor that is required for the activation and linkage of HOXA and arterial programs in the hemogenic endothelium and for establishing DLL4+CXCR4+ arterial hemogenic endothelium with definitive lympho-myeloid potential. These SOX17 effects are mediated through the activation of CDX2 and NOTCH signaling. |
| Databáze: | OpenAIRE |
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