Toona sinensis (leaf extracts) inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis in vascular endothelial cells
| Autor: | Ssu Ching Chen, Dong-Zong Hung, Hsin-Ling Yang, Hsin Ju Cho, Mei Tsun Wang, Lai Wang, Ming-Kuem Lin, Yueh-Hsiung Kuo, Wen-Hsin Lin, You-Cheng Hseu |
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| Rok vydání: | 2011 |
| Předmět: |
Vascular Endothelial Growth Factor A
Angiogenesis Toona sinensis Cell Line chemistry.chemical_compound In vivo Drug Discovery Humans Gallic acid Meliaceae Cell Proliferation Pharmacology Tube formation Neovascularization Pathologic biology Plant Extracts Cell Cycle biology.organism_classification Molecular biology Plant Leaves Endothelial stem cell Vascular endothelial growth factor Chorioallantoic membrane Biochemistry chemistry Endothelium Vascular |
| Zdroj: | Journal of Ethnopharmacology. 134:111-121 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2010.11.058 |
| Popis: | Aim of the study Toona sinensis is well known as a traditional Chinese medicine; also, it has been shown to exhibit anticancer and anti-inflammatory effects. This study was aimed at evaluating the anti-angiogenesis effect of the aqueous extracts of Toona sinensis (TS extracts) or gallic acid, a major component of TS extracts, against both VEGF-induced EA.hy 926 and human umbilical vein endothelial cells (HUVECs). Materials and methods Anti-proliferative activity of TS extracts or gallic acid, was determined against EA.hy 926 and HUVECs by trypan blue exclusion method. Invasion, tube formation and chick chorioallantoic membrane assay were carried out to determine the in vitro and in vivo anti-angiogenic effects. Results Non-cytotoxic concentration of TS extracts (50–100 μg/mL) and gallic acid (5 μg/mL) inhibited the proliferation of VEGF-stimulated EA.hy 926 and HUVECs. Inhibitory effects of TS extracts and gallic acid on angiogenesis were assessed by VEGF-induced migration/invasion and capillary-like tube formation by EA.hy 926 and HUVECs. Additionally, gelatin zymography assays showed that TS extracts and gallic acid suppressed the activity of metalloproteinase (MMP)-9 and MMP-2 activated by VEGF. In vivo , TS extracts and gallic acid strongly suppressed neovessel formation in the chorioallantoic membrane of chick embryos. Flow cytometry analyses and Western blot demonstrated that treatment with TS extracts and gallic acid induced G 0 /G 1 arrest in VEGF-stimulated EA.hy 926 cells via a reduction in the amounts of cyclin D1, cyclin E, CDK4, hyperphosphorylated retinoblastoma protein (pRb), VEGFR-2, and eNOS. Conclusions These results support an anti-angiogenic activity of Toona sinensis that may contribute critically to its cancer and inflammation chemopreventive potentials. |
| Databáze: | OpenAIRE |
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