An exceptionally long CA-repeat in the core promoter of SCGB2B2 links with the evolution of apes and Old World monkeys
| Autor: | M. Nikkhah, Maryam Rezazadeh, H.R. Khorram Khorshid, Mina Ohadi, Akbar Biglarian |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Old World biology Hominidae Bonobo Cercopithecidae Promoter General Medicine Old World monkey Secretoglobins biology.organism_classification Evolution Molecular 03 medical and health sciences 030104 developmental biology Cell culture Gene expression Animals Humans Dinucleotide Repeats Promoter Regions Genetic Gene |
| Zdroj: | Gene. 576:109-114 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/j.gene.2015.09.070 |
| Popis: | We have recently reported a genome-scale catalog of human protein-coding genes that contain "exceptionally long" STRs (≥6-repeats) in their core promoter, which may be of selective advantage in this species. At the top of that list, SCGB2B2 (also known as SCGBL), contains one of the longest CA-repeat STRs identified in a human gene core promoter, at 25-repeats. In the study reported here, we analyzed the conservation status of this CA-STR across evolution. The functional implication of this STR to alter gene expression activity was also analyzed in the HEK-293 cell line. We report that the SCGB2B2 core promoter CA-repeat reaches exceptional lengths, ranging from 9- to 25-repeats, across Apes (Hominoids) and the Old World monkeys (CA>2-repeats were not detected in any other species). The longest CA-repeats and highest identity in the SCGB2B2 protein sequence were observed between human and bonobo. A trend for increased gene expression activity was observed from the shorter to the longer CA-repeats (p |
| Databáze: | OpenAIRE |
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