Chondrocyte-seeded hydroxyapatite for repair of large articular cartilage defects. A pilot study in the goat
| Autor: | Pieter Buma, G.N. Homminga, René P. H. Veth, W.B. van den Berg, J.L.C. van Susante |
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| Rok vydání: | 1998 |
| Předmět: |
Herstel van kraakbeenlesies met in vitro gekweekte chondrocyten
Cartilage Articular Repair of cartilage with in vitro cultured chondrocytes Time Factors Materials science Cell Survival Surface Properties Biophysics De effecten van cytokines en groeifactoren op het chondrocytmetabolisme Pilot Projects Bioengineering Fibrin Tissue Adhesive Matrix (biology) Chondrocyte Bone remodeling Biomaterials The effects of cytokines and growth factors on chondrocyte metabolism medicine Animals Fibrin glue Bioprosthesis Goats Cartilage Graft Survival Resorption Transplantation Durapatite surgical procedures operative medicine.anatomical_structure Mechanics of Materials Ceramics and Composites Rabbits Cancellous bone Biomedical engineering |
| Zdroj: | Biomaterials, 19, 2367-2374 Biomaterials, 19, pp. 2367-2374 |
| ISSN: | 0142-9612 2367-2374 |
| Popis: | The aim of this study was to evaluate the potential for restoration of a large cartilage defect in the goat knee with hydroxyapatite (HA) loaded with chondrocytes. Isolated chondrocytes were suspended in fibrin glue, seeded on top of the HA, and then the composite graft was implanted in the defect. After transplantation, cell behaviour, newly synthesised matrix and the HA-glue interface were assessed histologically after 2, 4, 12, 26 and 52 weeks. Special attention was paid to the incorporation process of HA in the subchondral bone and interactions between this biomaterial and the fibrin-glue-chondrocyte suspension. Chondrocytes in the glue proved to survive the transplantation procedure and produced new metachromatically stained matrix two weeks after implantation. The glue-cell suspension had penetrated the superficial porous structure of the HA. Four weeks after surgery, islands of hyaline-like cartilage were observed at the HA-glue interface. A layer of fibrous tissue was formed surrounding the HA graft, resulting in a relatively instable fixation of the HA in the defect. This instability of the graft in the defect, possibly together with early weight bearing, resulted in a gradual loss of the newly formed hyaline cartilage-like repair tissue. Progressive resorption of the HA occurred without any sign of active bone remodelling from the host site. One year after surgery part of the defect which extended down to the cancellous bone had been predominantly restored with newly formed lamellar bone. Only small HA remnants were still present at the bottom of the original defect. Resurfacing of the joint had occurred with fibrocartilaginous repair tissue. The absence of adequate fixation capacity of the HA near the joint space resulted in a relative instability of the graft with progressive resorption. Therefore, HA is not a suitable biomaterial to facilitate the repair of large articular cartilage defects. |
| Databáze: | OpenAIRE |
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