Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase
| Autor: | Noor-Hidayah Awang-Kechik, Aida-Nur-Ashikin Abd-Rahman, Negar-Shafiei Sabet, Saeid-Reza Doustjalali, Rohana Ahmad |
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| Rok vydání: | 2019 |
| Předmět: |
Saliva
Chromatography Chemistry Research Orthodontics 030206 dentistry Proteomics CIENCIAS MÉDICAS [UNESCO] 03 medical and health sciences 0302 clinical medicine Immune system medicine.anatomical_structure Incisor Liquid chromatography–mass spectrometry Phase (matter) Proteome UNESCO::CIENCIAS MÉDICAS medicine Biomarker (medicine) 030212 general & internal medicine General Dentistry |
| Zdroj: | Awang-Kechik, Noor-Hidayah ; Ahmad, Rohana ; Doustjalali, Saeid-Reza ; Sabet, Negar-Shafiei ; Abd Rahman, Aida Nur Ashikin. Liquid Chromatography Mass Spectrometry (LC-MS) analysis in determining the saliva protein of orthodontic patients during retention phase. En: Journal of Clinical and Experimental Dentistry, 11 3 2019: 269-274 RODERIC. Repositorio Institucional de la Universitat de Valéncia instname Journal of Clinical and Experimental Dentistry |
| Popis: | Background The biological responses involved during retention phase have been studied for many years but little is known about the effect of saliva proteome during retention phase of post-orthodontic treatment. This study aims to identify the protein profiles during retention phase in relation to biological processes involved by Liquid Chromatography Mass Spectrometry (LC-MS) approach. Material and methods A total of 5 ml of unstimulated saliva was collected from each subject (10 non-orthodontic patients and 15 post-orthodontic patients with 6-months retention phase). Samples were then subjected to LC-MS analysis. The expressed proteins were identified and compared between groups. Incisor irregularity for both maxilla and mandible were determined with Little's Irregularity Index at 6-months retention phase. Results 146 proteins and 135 proteins were expressed in control and 6-months retention phase group respectively. 15 proteins were identified to be co-expressed between groups. Immune system process was only detected in 6-months retention phase group. Detected protein in immune system process was identified as Tyrosine-protein kinase Tec. Statistical significant of incisor irregularity was only found in mandible at 6-months retention phase. Conclusions Our study suggests that immune system process protein which is Tyrosine-protein kinase Tec could be used as biomarker for prediction of stability during retention phase of post-orthodontic treatment. Key words:Orthodontics, proteomics, retention, LC-MS, saliva. |
| Databáze: | OpenAIRE |
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