Scarce evidence of yogurt lactic acid bacteria in human feces after daily yogurt consumption by healthy volunteers
| Autor: | Patricia Ruiz-Garbajosa, Victor Abraira, Raimundo García-Albiach, Daniel Bravo, Fernando Baquero, Francisco-Javier Yuste, Alejandra Montesi-Libois, Rafael Cantón, Rosa del Campo |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Streptococcus thermophilus Microbiological culture Colony Count Microbial Public Health Microbiology Applied Microbiology and Biotechnology Polymerase Chain Reaction Microbiology chemistry.chemical_compound Feces Double-Blind Method Lactobacillus Humans Food science Prospective Studies Human feces Lactobacillus delbrueckii Ecology biology Probiotics food and beverages Nucleic Acid Hybridization Lactobacillaceae biology.organism_classification Yogurt Lactic acid Culture Media chemistry Female Bacteria Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology. 71(1) |
| ISSN: | 0099-2240 |
| Popis: | In a double-blind prospective study including 114 healthy young volunteers, the presence in human feces of the yogurt organisms Lactobacillus delbrueckii and Streptococcus thermophilus after repeated yogurt consumption (15 days) was analyzed by culture, specific PCR, and DNA hybridization of total fecal DNA. Detection of yogurt lactic acid bacteria in total fecal DNA by bacterial culture and PCR assay was consistently negative. DNA compatible with yogurt bacteria was found by hybridization experiments in only 10 (10.52%) of 96 individuals after consumption of fresh yogurt and in 2 (2.10%) of 96 individuals after consumption of pasteurized yogurt ( P = 0.01). |
| Databáze: | OpenAIRE |
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