Increased expression of Na+/H+ exchanger in the injured renal tissues of focal glomerulosclerosis in rats
| Autor: | Akinori Nagashima, Takashi Ando, Seiya Okuda, Masaru Nakayama, Kyoichi Fukuda, Kiyoshi Tamaki, Harumichi Higashi, Masatoshi Fujishima |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Nephrology medicine.medical_specialty Sodium-Hydrogen Exchangers Time Factors medicine.drug_class Molecular Probe Techniques Kidney Amiloride Rats Sprague-Dawley Internal medicine medicine Animals RNA Messenger Glomerulosclerosis Focal Segmental business.industry Furosemide Glomerulosclerosis Sodium Dietary Hypertrophy Glomerular Hypertrophy medicine.disease Rats Disease Models Animal Sodium–hydrogen antiporter medicine.anatomical_structure Endocrinology Doxorubicin Potassium-sparing diuretic business medicine.drug |
| Zdroj: | Kidney International. 46:1635-1643 |
| ISSN: | 0085-2538 |
| Popis: | Increased expression of Na + /H + exchanger in the injured renal tissues of focal glomerulosclerosis in rats. The renal mRNA expression of Na + /H + exchanger (NHE) and the effects of NHE inhibitor, amiloride, on renal injury were investigated in adriamycin (ADR)-induced glomerulosclerosis model in rats, which progressively developed extensive glomerulosclerosis and interstitial fibrosis. NHE-1 mRNA from the cortex of the ADR rats progressively increased at weeks 4 and 8 and then peaked at week 16, which paralleled with the degree of glomerular sclerosis and interstitial fibrosis. The interstitial fibrosis in the ADR-rats was prevented by a daily administration of amiloride. A simultaneous analysis of the effects of a high salt diet on NHE-1 mRNA expression or renal injury was performed in the ADR rats at weeks 2 and 8. Renal or glomerular hypertrophy was observed in the control or ADR rats fed an 8% NaCl diet at week 2 and 8 compared to a 1% NaCl diet, while the NHE-1 mRNA expression was not up-regulated by an 8% NaCl diet at week 2. At week 8, the NHE-1 mRNA expression or glomerulosclerosis and interstitial fibrosis were enhanced in the ADR rats fed an 8% NaCl diet compared to a 1% NaCl diet. This histological aggravation by an 8% NaCl diet was prevented by a daily administration of amiloride but not by furosemide. In conclusion, the increased NHE-1 mRNA expression and the preventive effects of amiloride on the renal lesions suggest a potential importance of NHE in the progressive process of ADR-nephropathy. The high salt diet had a hypertrophic and destructive effect on kidney or glomeruli in the ADR rats. The hypertrophic effect of a high salt diet did not seem to be mediated through NHE activation. |
| Databáze: | OpenAIRE |
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