Nitroxide malonate methanofullerene as biomimetic model of interaction of nitroxide species with antioxidants
| Autor: | Valentina P. Gubskaya, E. N. Kochetkov, M. V. Gulenova, N. B. Mel’nikova, O. E. Zhil’tsova, I. A. Nuretdinov, G.M. Fazlleeva, V.M. Korobko, A.I. Poddel’sky |
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| Rok vydání: | 2015 |
| Předmět: |
Nitroxide mediated radical polymerization
Cytochrome Photochemistry Antioxidants chemistry.chemical_compound Colloid and Surface Chemistry Biomimetics Polymer chemistry Physical and Theoretical Chemistry Heme Monolayers Aqueous solution biology Chemistry Silica gel Cytochrome с Cytochrome c Electron Spin Resonance Spectroscopy Nitroxide malonate methanofullerene Surfaces and Interfaces General Medicine Models Theoretical Ascorbic acid Biomimetic model of nitroxide species Malonate biology.protein Nitrogen Oxides Spectrophotometry Ultraviolet Fullerenes Antioxidant Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 136:314-322 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2015.09.026 |
| Popis: | Bis-nitroxide malonate methanofullerene (NO)2-MF was studied as a biomimetic model of reduction-oxidation activity with natural compounds-cytochrome c (cyt c), dihydroquercetin (DHQ), ascorbic acid (AA) and synthetic drug-1-(β-oxyethyl)-4,6-dimethyl-1,2-dihydro-2-oxopyrimidine (xymedon(®)). (NO)2-MF may be used as the component of Langmuir monolayers on an aqueous subphase and as the adsorbate on silica gel. The activity of (NO)2-MF in the reaction with cyt c was compared with the effect of nitroxide species such as gaseous nitric oxide, 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) by using UV-vis and EPR-spectra. It has been shown, that iron(III) in cyt c(3+) under action (NO)2-MF was reduced up to iron(II), similar effect was observed under the influence of gaseous NO in aqueous solution, but reduction of iron(III) in heme cyt c was reversible in the presence of TEMPO. Therefore, the state of Fe-heme in cyt c can be used as the indicator of the interaction of cyt c with nitroxide species in vitro. The interaction of cyt c, DHQ, xymedon(®) with (NO)2-MF monolayers was confirmed by the increasing of limiting area А0 from 0.88 nm(2) up to 1.70 nm(2) of (NO)2-MF on the aqueous subphase, by the paramagnetism and UV-vis spectral data changes. These results can be explained by appearance of oxoammonium ion (NO(+))2-MF adlayers and monolayers. The antioxidant and regenerating effects were shown when treating wounds by xymedon(®) in the presence of additives (0.001%) of (NO)2-MF in the experiments on the rats. |
| Databáze: | OpenAIRE |
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