Homocysteine Induces Glial Reactivity in Adult Rat Astrocyte Cultures
| Autor: | André Quincozes-Santos, Adriano Martimbianco de Assis, Janaína Kolling, Tiago Marcon dos Santos, Camila Leite Santos, Aline Longoni, Bruna Bellaver, Angela T. S. Wyse, Yasmine Nonose, Larissa Daniele Bobermin |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Cell Survival Neuroscience (miscellaneous) medicine.disease_cause Antioxidants Superoxide dismutase 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Animals Viability assay Rats Wistar Homocysteine Cells Cultured Cerebral Cortex chemistry.chemical_classification Dose-Response Relationship Drug biology Glutathione peroxidase Age Factors Glutathione Rats Cell biology Heme oxygenase Oxidative Stress 030104 developmental biology medicine.anatomical_structure Endocrinology Neurology chemistry Astrocytes biology.protein Inflammation Mediators Neuroglia 030217 neurology & neurosurgery Homeostasis Oxidative stress Astrocyte |
| Zdroj: | Molecular Neurobiology. 55:1966-1976 |
| ISSN: | 1559-1182 0893-7648 |
| Popis: | Astrocytes are dynamic glial cells associated to neurotransmitter systems, metabolic functions, antioxidant defense, and inflammatory response, maintaining the brain homeostasis. Elevated concentrations of homocysteine (Hcy) are involved in the pathogenesis of age-related neurodegenerative disorders, such as Parkinson and Alzheimer diseases. In line with this, our hypothesis was that Hcy could promote glial reactivity in a model of cortical primary astrocyte cultures from adult Wistar rats. Thus, cortical astrocytes were incubated with different concentrations of Hcy (10, 30, and 100 μM) during 24 h. After the treatment, we analyzed cell viability, morphological parameters, antioxidant defenses, and inflammatory response. Hcy did not induce any alteration in cell viability; however, it was able to induce cytoskeleton rearrangement. The treatment with Hcy also promoted a significant decrease in the activities of Na+, K+ ATPase, superoxide dismutase (SOD), and glutathione peroxidase (GPx), as well as in the glutathione (GSH) content. Additionally, Hcy induced an increase in the pro-inflammatory cytokine release. In an attempt to elucidate the putative mechanisms involved in the Hcy-induced glial reactivity, we measured the nuclear factor kappa B (NFκB) transcriptional activity and heme oxygenase 1 (HO-1) expression, which were activated and inhibited by Hcy, respectively. In summary, our findings provide important evidences that Hcy modulates critical astrocyte parameters from adult rats, which might be associated to the aging process. |
| Databáze: | OpenAIRE |
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