Zucchini vein clearing disease is caused by several lineages within Pseudomonas syringae species complex
| Autor: | Caroline Lacault, Martial Briand, Armelle Darrasse, Marie-Agnès Jacques |
|---|---|
| Přispěvatelé: | Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Species complex Sequence analysis Phytopathology and phytopharmacy mécanisme de résistance Pseudomonas syringae Plant Science 01 natural sciences Genome bacterial seedborne disease Analyse de séquance multilocus (MLSA) 03 medical and health sciences Cucurbita pepo cucurbits [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Whole genome sequencing Genetics bactérie phytopathogène Vegetal Biology biology collection bactérienne Strain (biology) Microbiology and Parasitology souche pathogène biology.organism_classification Phytopathologie et phytopharmacie Microbiologie et Parasitologie maladie des plantes [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy Agricultural sciences 030104 developmental biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Pathovar génétique bactérienne organisme transmissible par semence Agronomy and Crop Science Biologie végétale Sciences agricoles 010606 plant biology & botany |
| Zdroj: | Phytopathology, preprint. (2020) Phytopathology Phytopathology, American Phytopathological Society, 2020, ⟨10.1094/PHYTO-07-19-0266-R⟩ |
| ISSN: | 0031-949X |
| DOI: | 10.1094/PHYTO-07-19-0266-R⟩ |
| Popis: | Zucchini (Cucurbita pepo) is worldwide affected by Pseudomonas syringae, inducing vein clearing, stunting, and necroses during plantlet development. A collection of 58 P. syringae strains isolated from diseased zucchini plantlets was characterized by multilocus sequence analysis (MLSA). A subset of 23 strains responsible for vein clearing of zucchini (VCZ) was evaluated for pathogenicity on zucchini, and their genomes were sequenced. The host range of six VCZ strains was evaluated on 11 cucurbit species. Most VCZ strains belong to clades 2a and 2b-a within phylogroup 2 of P. syringae species complex and are closely related to other strains previously isolated from cucurbits. Genome analyses revealed diversity among VCZ strains within each clade. One main cluster, once referred to by the invalid pathovar name (peponis), gathers VCZ strains presenting a narrow host range including zucchini and squashes. Other VCZ strains present a large host range including zucchini, squashes, cucumber, melons, and in some cases watermelon. The VCZ strain pathogenic features are strongly associated with type III effector repertoires. The presence of avrRpt2 and absence of hopZ5 are associated with a narrow host range, whereas the presence of hopZ5 and absence of avrRpt2 are most generally associated with a large host range. To better detect the different clusters identified with whole genome sequence and pathogenicity analyses, we used a specific-k-mers approach to refine the MLSA scheme. Using this novel MLSA scheme to type P. syringae isolates from diseased cucurbits would give insight into distribution of worldwide strains and origin of epidemics. |
| Databáze: | OpenAIRE |
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