Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi-harmonic content from cells and microspheres
| Autor: | Patrick Jenkins, Jessica P. Houston, Mark A. Naivar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
General Physics and Astronomy
CHO Cells General Biochemistry Genetics and Molecular Biology Light scattering Article Fluorescence Flow cytometry symbols.namesake Optics Cricetulus medicine Animals Scattering Radiation General Materials Science Computer Simulation medicine.diagnostic_test Fourier Analysis Chemistry business.industry Lasers General Engineering General Chemistry Square wave Equipment Design Models Theoretical Flow Cytometry Microspheres Nonlinear Dynamics Fourier analysis Modulation Frequency domain symbols Harmonic business Biological system |
| Popis: | Illustration of a flow cytometer capable of capturing multiple fluorescence lifetime measurements; creating potential for multi-parametric, time-resolved signals to be captured for every color channel. Flow cytometry is a powerful means for in vitro cellular analyses where multi-fluorescence and multi-angle light scattering can indicate unique biochemical or morphological features of single cells. Yet, to date, flow cytometry systems have lacked the ability to capture complex fluorescence dynamics due to the transient nature of flowing cells. In this contribution we introduce a simple approach for measuring multiple fluorescence lifetimes from a single cytometric event. We leverage square wave modulation, Fourier analysis, and high frequency digitization and show the ability to resolve more than one fluorescence lifetime from fluorescently-labelled cells and microspheres. |
| Databáze: | OpenAIRE |
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