Oxidation of 2-Cys-peroxiredoxins by Arachidonic Acid Peroxide Metabolites of Lipoxygenases and Cyclooxygenase-2
| Autor: | Kornelia Edes, Pauline Cordray, Frank A. Fitzpatrick, Philip J. Moos, Kelly Doyle |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Cell signaling
Lipoxygenase Biochemistry Peroxide Catalysis Gene Expression Regulation Enzymologic Cell Line chemistry.chemical_compound Humans Cysteine Lipoxygenase Inhibitors Molecular Biology Arachidonic Acid biology Chemistry Peroxiredoxins Cell Biology respiratory system Peroxides Enzyme Activation Cyclooxygenase 2 biology.protein Arachidonic acid Cyclooxygenase Peroxiredoxin Oxidation-Reduction Protein Binding Peroxidase |
| Zdroj: | Journal of Biological Chemistry. 282:32623-32629 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m704369200 |
| Popis: | Human peroxiredoxins serve dual roles as anti-oxidants and regulators of H2O2-mediated cell signaling. The functional versatility of peroxiredoxins depends on progressive oxidation of key cysteine residues. The sulfinic or sulfonic forms of peroxiredoxin lose their peroxidase activity, which allows cells to accumulate H2O2 for signaling or pathogenesis in inflammation, cancer, and other disorders. We report that arachidonic acid lipid hydroperoxide metabolites of 5-, 12-, 15-lipoxygenase-1, and cyclooxygenase-2 oxidize the 2-Cys-peroxiredoxins 1, 2, and 3 to their sulfinic and sulfonic forms. When added exogenously to cells, 5-, 12- and 15-hydroperoxy-eicosatetraenoic acids also over-oxidized peroxiredoxins. Our results suggest that lipoxygenases and cyclooxygenases may affect 2-Cys peroxiredoxin signaling, analogous to NADPH oxidases in the “floodgate” model (Wood, Z. A., Poole, L. B, and Karplus P. A. (2003) Science 300, 600–653). Peroxiredoxin-dependent mechanisms may modulate the receptor-dependent actions of autocoids derived from cellular lipoxygenase and cyclooxygenase catalysis. |
| Databáze: | OpenAIRE |
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