Identification and Quantification of β-Sitosterol β-d-Glucoside of an Ethanolic Extract Obtained by Microwave-Assisted Extraction from Agave angustifolia Haw
| Autor: | Martha Lucía Arenas Ocampo, Sandra Victoria Ávila-Reyes, Herminia López-Salazar, Brenda Hildeliza Camacho-Díaz, Ma Dolores Pérez-García, Antonio Ruperto Jiménez-Aparicio, Manasés González-Cortazar |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Agave angustifolia Haw Pharmaceutical Science Mass spectrometry 01 natural sciences Microwave assisted mae Article Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound Agave Glucoside Dry weight lcsh:Organic chemistry β-sitosterol β-d-glucoside Spectroscopy Fourier Transform Infrared Drug Discovery Maceration (wine) Physical and Theoretical Chemistry Microwaves Chromatography Ethanol biology Plant Extracts 010405 organic chemistry Chemistry 010401 analytical chemistry Organic Chemistry agave angustifolia haw Reference Standards biology.organism_classification Sitosterols Thin-layer chromatography 0104 chemical sciences Solutions Chemistry (miscellaneous) Molecular Medicine Chromatography Thin Layer |
| Zdroj: | Molecules, Vol 24, Iss 21, p 3926 (2019) Molecules Volume 24 Issue 21 |
| ISSN: | 1420-3049 |
| Popis: | &beta sitosterol &beta d-glucoside (BSSG) was extracted from &ldquo piñ a&rdquo of the Agave angustifolia Haw plant by microwave-assisted extraction (MAE) with a KOH solution such as a catalyst and a conventional maceration method to determine the best technique in terms of yield, extraction time, and recovery. The quantification and characterization of BSSG were done by high-performance thin layer chromatography (HPTLC), Fourier-transform infrared spectroscopy (FT-IR), and high-performance liquid chromatography&minus electrospray ionization&minus mass spectrometry (HPLC-ESI-MS). With an extraction time of 5 s by MAE, a higher amount of BSSG (124.76 mg of &beta d-glucoside/g dry weight of the extract) than those for MAE extraction times of 10 and 15 s (106.19 and 103.97 mg/g dry weight respectively) was shown. The quantification of BSSG in the extract obtained by 48 h of conventional maceration was about 4&ndash 5 times less (26.67 mg/g dry weight of the extract) than the yields reached by the MAE treatments. MAE achieved the highest amount of BSSG, in the shortest extraction time while preserving the integrity of the compound&rsquo s structure. |
| Databáze: | OpenAIRE |
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