Murine maternal dietary restriction affects neural Humanin expression and cellular profile
| Autor: | Sherin U. Devaskar, Xin Ye, Claire Baldauf, Bo-Chul Shin, Kuk-Wha Lee, Young Eun Ko, Monica Sondhi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
AB_561053 AB_631729 [RRID] AB_477629 [RRID] SCR_002140 AB_2313773 [RRID] SCR_013673 Mice 0302 clinical medicine AB_10950489 Infant Mortality Psychology AB_477662 [RRID] SCR_014246 Cerebral Cortex Neurons AB_2340375 [RRID] nutrient restriction AB_650336 Fetal Growth Retardation Glial fibrillary acidic protein neurodevelopment SCR_002798 AB_2336177 AB_650336 [RRID] Intracellular Signaling Peptides and Proteins AB_2336177 [RRID] SCR_017411 [RRID] Cerebral cortex AB_2313584 [RRID] AB_2340432 [RRID] AB_793998 AB_2286684 [RRID] medicine.medical_specialty AB_443209 [RRID] Pediatric Research Initiative intrauterine growth restriction SCR_014246 [RRID] Offspring 1.1 Normal biological development and functioning Calorie restriction AB_2313584 Article 03 medical and health sciences AB_10695870 fetal brain Preterm AB_2340375 AB_305670 RRID AB_443209 AB_1619688 [RRID] SCR_017411 AB_305670 [RRID] AB_732011 [RRID] SCR_002368 [RRID] medicine.disease AB_1619688 Humanin AB_561053 [RRID] 030104 developmental biology Endocrinology AB_532250 [RRID] AB_477662 030217 neurology & neurosurgery AB_793998 [RRID] 0301 basic medicine AB_631729 AB_532250 Intrauterine growth restriction Reproductive health and childbirth Low Birth Weight and Health of the Newborn SCR_013673 [RRID] AB_1616567 AB_772206 reproductive and urinary physiology Pediatric biology medicine.anatomical_structure Neurological embryonic structures Female SCR_002140 [RRID] AB_732011 SCR_002368 Cellular and Molecular Neuroscience AB_2286684 Underpinning research Internal medicine Glial Fibrillary Acidic Protein medicine Animals AB_10695870 [RRID] Caloric Restriction Fetus Neurology & Neurosurgery AB_2313773 AB_2340432 Neurosciences Maternal Nutritional Physiological Phenomena Perinatal Period - Conditions Originating in Perinatal Period AB_772206 [RRID] SCR_002798 [RRID] AB_10950489 [RRID] Doublecortin AB_477629 biology.protein AB_1616567 [RRID] |
| Zdroj: | J Neurosci Res Journal of neuroscience research, vol 98, iss 5 |
| ISSN: | 1097-4547 |
| Popis: | To understand the cellular basis for the neurodevelopmental effects of intrauterine growth restriction (IUGR), we examined the global and regional expression of various cell types within murine (Mus musculus) fetal brain. Our model employed maternal calorie restriction to 50% daily food intake from gestation day 10-19, producing IUGR offspring. Offspring had smaller head sizes with larger head:body ratios indicating a head sparing IUGR effect. IUGR fetuses at embryonic day 19 (E19) had reduced nestin (progenitors), β-III tubulin (immature neurons), Glial fibrillary acidic protein (astrocytes), and O4 (oligodendrocytes) cell lineages via immunofluorescence quantification and a 30% reduction in cortical thickness. No difference was found in Bcl-2 or Bax (apoptosis) between controls and IUGR, though qualitatively, immunoreactivity of doublecortin (migration) and Ki67 (proliferation) was decreased. In the interest of examining a potential therapeutic peptide, we next investigated a novel pro-survival peptide, mouse Humanin (mHN). Ontogeny examination revealed highest mHN expression at E19, diminishing by postnatal day 15 (P15), and nearly absent in adult (3months). Subanalysis by sex at E19yielded higher mHN expression among males during fetal life, without significant difference between sexes postnatally. Furthermore, femaleIUGR mice at E19had a greater increase in cortical mHN versus the male fetus over their respective controls. We conclude that maternal dietary restriction-associated IUGR interferes with neural progenitors differentiating into the various cellular components populating the cerebral cortex, and reduces cerebral cortical size. mHN expression is developmental stage and sex specific, with IUGR, particularly in the females, adaptively increasing its expression toward mediating a pro-survival approach against nutritional adversity. |
| Databáze: | OpenAIRE |
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