Behavior of the meat-borne bacterium Lactobacillus sakei during its transit through the gastrointestinal tracts of axenic and conventional mice
| Autor: | Stéphane Chaillou, Sébastien Blugeon, Monique Zagorec, Fabrizio Chiaramonte, Philippe Langella |
|---|---|
| Přispěvatelé: | Unité de recherche Flore Lactique et Environnement Carné (UFLEC), Institut National de la Recherche Agronomique (INRA), Unité de recherche d'Écologie et Physiologie du Système Digestif (UEPSD), EC EST ( MEST-2-CT-2004-514428), Zagorec, Monique |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Colony Count Microbial adaptation souris medicine.disease_cause Applied Microbiology and Biotechnology Feces Mice RNA Ribosomal 16S Lactobacillus souche Cluster Analysis Axenic Phylogeny Human feces lactic-acid bacteria diversity expression growth strain identification mouse gut human feces escherichia-coli 0303 health sciences education.field_of_study Ecology biology Strain (chemistry) excrément food and beverages escherichia coli Biotechnology DNA Bacterial Meat bactérie lactique Molecular Sequence Data Population DNA Ribosomal Microbiology 03 medical and health sciences intestin medicine Animals Humans education Escherichia coli diversité 030304 developmental biology 030306 microbiology Sequence Analysis DNA biology.organism_classification croissance Lactobacillus sakei Gastrointestinal Tract Genes Bacterial Food Microbiology Microscopy Electron Scanning Food Science |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2009, 75 (13), pp.4498-4505. ⟨10.1128/AEM.02868-08⟩ Applied and Environmental Microbiology 13 (75), 4498-4505. (2009) |
| ISSN: | 0099-2240 1098-5336 |
| DOI: | 10.1128/AEM.02868-08⟩ |
| Popis: | A Lactobacillus sakei strain named FLEC01 was isolated from human feces and characterized genotypically. Comparison of the genetic features of this strain with those of both the meat-borne L. sakei strain 23K and another human isolate, LTH5590, showed that they belong to different but closely related clusters. The three L. sakei strains did not persist and only transited through the gastrointestinal tracts (GITs) of conventional C3H/HeN mice. In contrast, they all colonized the GITs of axenic mice and rapidly reached a population of 10 9 CFU/g of feces, which remained stable until day 51. Five days after mice were fed, a first subpopulation, characterized by small colonies, appeared and reached 50% of the total L. sakei population in mice. Fifteen to 21 days after feeding, a second subpopulation, characterized by rough colonies, appeared. It coexisted with the two other populations until day 51, and its cell shapes were also affected, suggesting a dysfunction of the cell division or cell wall. No clear difference between the behaviors of the meat-borne strain and the two human isolates in both conventional and axenic mice was observed, suggesting that L. sakei is a food-borne bacterium rather than a commensal one and that its presence in human feces originates from diet. Previous observations of Escherichia coli strains suggest that the mouse GIT environment could induce mutations to increase their survival and colonization capacities. Here, we observed similar mutations concerning a food-grade gram-positive bacterium for the first time. |
| Databáze: | OpenAIRE |
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