Whole Genomic DNA Methylation Profiling of CpG Sites in Promoter Regions of Dorsal Root Ganglion in Diabetic Neuropathic Pain Mice
| Autor: | Danmin Shen, Weihua Cui, Ting Lan, Yingzi Chong, Jing Liu, Peipei Wang, Fei Yang, Wen Chen, Qian Li, Qingyu Sun, Tingting Hu, Yurui Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Bisulfite sequencing Biology 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Diabetic Neuropathies Ganglia Spinal Gene expression Animals Promoter Regions Genetic Gene Whole Genome Sequencing Promoter General Medicine Methylation DNA Methylation Molecular biology Mice Inbred C57BL 030104 developmental biology DNA demethylation CpG site DNA methylation Neuralgia CpG Islands 030217 neurology & neurosurgery |
| Zdroj: | Journal of molecular neuroscience : MN. 71(12) |
| ISSN: | 1559-1166 |
| Popis: | DNA methylation and demethylation play an important role in neuropathic pain. In general, DNA methylation of CpG sites in the promoter region impedes gene expression, whereas DNA demethylation contributes to gene expression. Here, we evaluated the methylation status of CpG sites in genomic DNA promoter regions in dorsal root ganglions (DRGs) of diabetic neuropathic pain (DNP) mice. In our research, streptozotocin (STZ) was intraperitoneally injected into mice to construct DNP models. The DNP mice showed higher fasting blood glucose (above 11.1 mmol/L), lower body weight, and mechanical allodynia than control mice. Whole-genome bisulfite sequencing (WGBS) revealed an altered methylation pattern in CpG sites in the DNA promoter regions in DRGs of DNP mice. The results showed 376 promoter regions with hypermethylated CpG sites and 336 promoter regions with hypomethylated CpG sites. In addition, our data indicated that altered DNA methylation occurs primarily on CpG sites in DNA promoter regions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that differentially methylated CpG sites annotated genes were involved in activities of the nervous and sensory systems. Enrichment analysis indicated that genes in these pathways contributed to diabetes or pain. In conclusion, our study enriched the role of DNA methylation in DNP. |
| Databáze: | OpenAIRE |
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