Comparative Proteomic and Transcriptomic Analysis of Follistatin-Induced Skeletal Muscle Hypertrophy

Autor: Stéphanie Devassine, Caroline Barbé, Fabrice Bray, Marine Gueugneau, Christian Rolando, Pascale Lause, Caroline Tokarski, Jean-Paul Thissen
Přispěvatelé: Pole of Endocrinology, Diabetes and Nutrition, Université Catholique de Louvain (UCL), Centre National de la Recherche Scientifique (CNRS), Université de Lille, FR 2638, Institut Eugene Michel Chevreul (FR 2638), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Université Catholique de Louvain = Catholic University of Louvain (UCL), Institut Michel Eugène Chevreul - FR 2638 (IMEC), Université d'Artois (UA)-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université d'Artois (UA)-Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Catholique de Louvain ( UCL ), Centre National de la Recherche Scientifique ( CNRS ), Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes )
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Proteomics
0301 basic medicine
Genetically modified mouse
medicine.medical_specialty
Mice
Transgenic
Myostatin
Biochemistry
Transcriptome
Mice
03 medical and health sciences
skeletal muscle hypertrophy
Muscular Diseases
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology
Internal medicine
medicine
follistatin
Animals
Humans
Regeneration
Muscle
Skeletal
Calcium signaling
mass spectrometry
biology
Gene Expression Profiling
Regeneration (biology)
Skeletal muscle
Hypertrophy
General Chemistry
musculoskeletal system
Cell biology
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Gene Expression Regulation
myostatin
GDF11
biology.protein
[ CHIM.ANAL ] Chemical Sciences/Analytical chemistry
microarray
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Follistatin
Zdroj: Journal of Proteome Research
Journal of Proteome Research, American Chemical Society, 2017, 16 (10), pp.3477-3490. ⟨10.1021/acs.jproteome.7b00069⟩
Journal of Proteome Research, 2017, 16 (10), pp.3477-3490. ⟨10.1021/acs.jproteome.7b00069⟩
Journal of Proteome Research, American Chemical Society, 2017, 16 (10), pp.3477-3490. 〈10.1021/acs.jproteome.7b00069〉
ISSN: 1535-3893
1535-3907
DOI: 10.1021/acs.jproteome.7b00069⟩
Popis: Skeletal muscle, the most abundant body tissue, plays vital roles in locomotion and metabolism. Myostatin is a negative regulator of skeletal muscle mass. In addition to increasing muscle mass, Myostatin inhibition impacts muscle contractility and energy metabolism. To decipher the mechanisms of action of the Myostatin inhibitors, we used -proteomic and transcriptomic approaches to investigate the changes induced in skeletal muscles of transgenic mice overexpressing Follistatin, a physiological Myostatin inhibitor. Our proteomic workflow included a fractionation step to identify weakly expressed proteins and a comparison of fast versus slow muscles. Functional annotation of altered proteins supports the phenotypic changes induced by Myostatin inhibition, including modifications in energy metabolism, fiber type, insulin and calcium signaling, as well as membrane repair and regeneration. Less than 10% of the differentially expressed proteins were found to be also regulated at the mRNA level but the Biological Process annotation, and the KEGG pathways analysis of transcriptomic results shows a great concordance with the proteomic data. Thus this study describes the most extensive omics analysis of muscle overexpressing Follistatin, providing molecular-level insights to explain the observed muscle phenotypic changes.
Databáze: OpenAIRE
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