SARS-Cov-2RNA found on particulate matter of Bergamo in Northern Italy: First evidence
| Autor: | Pierluigi Barbieri, Prisco Piscitelli, Leonardo Setti, Jolanda Palmisani, Alberto Pallavicini, Massimo Borelli, Libera Clemente, Francesco Fontana, Gianluigi de Gennaro, Fabrizio Passarini, Maurizio Ruscio, Valentina Torboli, Alessandro Miani, Alessia Di Gilio, Maria Grazia Perrone |
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| Přispěvatelé: | Setti, L., Passarini, F., De Gennaro, G., Barbieri, P., Perrone, M. G., Borelli, M., Palmisani, J., Di Gilio, A., Torboli, V., Fontana, F., Lanzetti, Clemente, Pallavicini, A., Ruscio, M., Piscitelli, P., Miani, A., Setti L., Passarini F., De Gennaro G., Barbieri P., Perrone M.G., Borelli M., Palmisani J., Di Gilio A., Torboli V., Fontana F., Clemente L., Pallavicini A., Ruscio M., Piscitelli P., Miani A. |
| Rok vydání: | 2020 |
| Předmět: |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Pneumonia Viral Air pollution 010501 environmental sciences Biology 01 natural sciences Biochemistry Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Molecular marker 030212 general & internal medicine Gene 0105 earth and related environmental sciences General Environmental Science Betacoronaviru Pandemic Coronavirus Infection SARS-CoV-2 COVID-19 RNA Particulates biology.organism_classification Virology Northern italy Particulate matter Italy chemistry RNA Viral Particulate Matter RNA extraction Betacoronavirus Human |
| Zdroj: | Environmental Research |
| ISSN: | 0013-9351 |
| DOI: | 10.1016/j.envres.2020.109754 |
| Popis: | Background The burden of COVID-19 was extremely severe in Northern Italy, an area characterized by high concentrations of particulate matter (PM), which is known to negatively affect human health. Consistently with evidence already available for other viruses, we initially hypothesized the possibility of SARS-CoV-2 presence on PM, and we performed a first experiment specifically aimed at confirming or excluding this research hyphotesys. Methods We have colelcted 34 PM10 samples in Bergamo area (the epicenter of the Italian COVID-19 epidemic) by using two air samplers over a continuous 3-weeks period. Filters were properly stored and underwent RNA extraction and amplification according to WHO protocols in two parallel blind analyses performed by two different authorized laboratories. Up to three highly specific molecular marker genes (E, N, and RdRP) were used to test the presence of SARS-CoV-2 RNA on particulate matter. Results The first test showed positive results for gene E in 15 out of 16 samples, simultaneously displaying positivity also for RdRP gene in 4 samples. The second blind test got 5 additional positive results for at least one ofthe three marker genes. Overall, we tested 34 RNA extractions for the E, N and RdRP genes, reporting 20 positive results for at least one of the three marker genes, with positivity separately confirmed for all the three markers. Control tests to exclude false positivities were successfully accomplished. Conclusion This is the first evidence that SARS-CoV-2 RNA can be present on PM, thus suggesting a possible use as indicator of epidemic recurrence. Highlights • COVID-19 burden seems more severe in areas with high concentrations of PM. • Particulate matter is already known to have negative effects on human health. • This is the first evidence that SARS-CoV-2RNA can be found on particulate matter. |
| Databáze: | OpenAIRE |
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