Raman Spectroscopic Evaluation of the Effects of Diet and Lipid-Lowering Therapy on Atherosclerotic Plaque Development in Mice
| Autor: | Oscar L. Volger, Louis M. Havekes, Sweder W.E van de Poll, Hans M.G. Princen, Gerwin J. Puppels, Arnoud van der Laarse, Dianne J.M. Delsing, J. Wouter Jukema, Tjeerd J. Römer, Tom C. Bakker Schut |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Aortic arch Apolipoprotein E Pathology medicine.medical_specialty Apolipoprotein B Arteriosclerosis Atorvastatin Apolipoprotein E3 Mice Transgenic Spectrum Analysis Raman Mice chemistry.chemical_compound symbols.namesake Apolipoproteins E In vivo medicine.artery medicine Animals Pyrroles Aorta biology business.industry Cholesterol Anticholesteremic Agents Calcinosis Reproducibility of Results medicine.disease Treatment Outcome chemistry Heptanoic Acids Disease Progression biology.protein symbols Diet Atherogenic Female lipids (amino acids peptides and proteins) Cardiology and Cardiovascular Medicine business Raman spectroscopy medicine.drug Calcification |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 21:1630-1635 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/hq1001.096651 |
| Popis: | Quantitative characterization of atherosclerotic plaque composition with standard histopathological methods remains limited to sectioned plaques. Raman spectroscopy enables nondestructive quantification of atherosclerotic plaque composition. We used Raman spectroscopy to study the effects of diet and lipid-lowering therapy on plaque development in apolipoprotein (APO) E*3-Leiden transgenic mice. Raman spectra were obtained over the full width and entire length of the ascending aorta and aortic arch. Spectra were modeled to calculate the relative dry weights of cholesterol and calcium salts, and quantitative maps of their distribution were created. In male mice (n=20) that received a high-fat/high-cholesterol (HFC) diet for 0, 2, 4, or 6 months, Raman spectroscopy showed good correlation between cholesterol accumulation and total serum cholesterol exposure ( r ≈0.87, P P |
| Databáze: | OpenAIRE |
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