Obtaining mutant fungal strains of Aspergillus niger with high production of fructooligosaccharides (FOS) using ultraviolet light irradiation
| Autor: | Javier Moreno, Boris Gutarra Castillo., Juan Cisneros de la Cruz, Marina DÃez-Municio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine 030106 microbiology Mutant 01 natural sciences Applied Microbiology and Biotechnology law.invention 03 medical and health sciences purl.org/pe-repo/ocde/ford#1.06.00 [http] law 010608 biotechnology Fructooligosaccharides Genetics Extracellular Ultraviolet light Irradiation Molecular Biology Polymerase chain reaction Mycelium Aspergillus biology Aspergillus niger biology.organism_classification Biochemistry Mutagenesis Agronomy and Crop Science Biotechnology |
| Zdroj: | Universidad Continental Repositorio Institucional-Continental CONTINENTAL-Institucional instacron:CONTINENTAL |
| Popis: | A strain of Aspergillus niger PR-142 native to northern coast of Peru was subjected to successive processes of mutagenesis by ultraviolet light (UV) irradiation at 253.7 nm to increase the production of fructooligosaccharides (FOS). An initial selection was made by considering the mutants with increased invertase activity followed by the measurement of β-fructosyltransferase (FTase) activity both in mycelium and extracellular environment. Five selected mutants, which showed increased values of mycelium invertase activity (ranging from 101 to 128% as compared to the parent strain) at 40°C and sodium dodecylsulfate 0.15 (w/v), were grown in a fermentative medium in 50 mL conical tubes on a rotary shaker, and their FTase activity was determined. The 6-M69 mutant showed the most active mycelium activity of 1.5 fold as compared to the parent strain. When the same reaction was performed between 1 to 4 h, at the 3rd h, the mycelium FTase activity significantly increased up to 7 and 3 times in the mutant and parental strain, respectively. Finally, 4 mutants and the parental PR-142 were genetically characterized using inter simple sequence repeat polymerase chain reaction (ISSR-PCR) molecular markers. This analysis showed a significant 33% polymorphic bands between the parent and mutant markers, and 20 bands were unique to the mutants. Key words: Aspergillus, mutagenesis, β-fructosyltransferase, fructooligosaccharides, inter simple sequence repeat polymerase chain reaction (ISSR-PCR). |
| Databáze: | OpenAIRE |
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