An electrochemical adaptation of Ellman’s test

Autor:	Olga Nekrassova, Sarah Threlfell, Nathan S. Lawrence, James Davis, Richard G. Compton, Andrew J. Wain, Paul C. White, Geraldine Hignett
Rok vydání:	2002
Předmět:	chemistry.chemical_classification Detection limit Chromatography Chemistry Analytical chemistry Electrochemistry Biochemistry Analytical Chemistry Linear range Thiol Environmental Chemistry Cyclic voltammetry Selectivity Quantitative analysis (chemistry) Spectroscopy Cysteine
Zdroj:	The Analyst. 127:797-802
ISSN:	1364-5528 0003-2654
Popis:	The electrochemically initiated reaction of thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined. The detection methodology has been proven to be robust with a linear range for cysteine from 2-120 microM, a limit of detection of 1.17 microM and has shown selectivity against a wide range of potential interferences. The efficiency of the methodology has been examined in the determination and recovery of thiol species in three growth tissue media, which contain a number of common biological interferences.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c663785bc3151f430f870e2762a8cfb5 https://doi.org/10.1039/b202780c Zobrazit plný text záznamu