Induction of apoptosis and bcl-2 expression in acute lymphoblastic leukaemia and non-Hodgkin's lymphoma in children
| Autor: | Barbara Hajto, Anna Pituch-Noworolska, Kinga Klus, Walentyna Balwierz |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Vincristine Myeloid Necrosis Adolescent medicine.medical_treatment Antineoplastic Agents Apoptosis Bone Marrow Cells Dexamethasone hemic and lymphatic diseases Humans Medicine ATP Binding Cassette Transporter Subfamily B Member 1 Child Chemotherapy Tumor Necrosis Factor-alpha business.industry Lymphoma Non-Hodgkin Granulocyte-Macrophage Colony-Stimulating Factor Hematology Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Non-Hodgkin's lymphoma Lymphoma medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Child Preschool Immunology Cancer research Female Tumor necrosis factor alpha Lymph Nodes medicine.symptom business medicine.drug |
| Zdroj: | Haematologia. 31:191-207 |
| ISSN: | 1568-5594 0017-6559 |
| DOI: | 10.1163/15685590152763737 |
| Popis: | bcl-2 expression is associated with the expression of the multidrug resistance molecule (p-gp) and the resistance of leukaemia cells to the induction of apoptosis. The activity of p-gp is the main mechanism of resistance of leukaemia cells to chemotherapy. This study assessed the induction of apoptosis of acute lymphoblastic leukaemia (ALL) and non-Hodgkin's lymphoma (NHL) blastic cells following in vitro treatment with dexamethasone (DXM), vincristine (VCR), and tumour necrosis factor (TNF) in relation to the expression of bcl-2 and p-gp. Common ALL (cALL; n = 24 patients), common ALL with co-expression of myeloid antigens (cALL + My; n = 9), ALL-T (n = 9), and NHL [n = 6 (T type, n = 2; B type, n = 4)] were included. The expression of bcl-2 and p-gp and apoptosis were assayed by flow cytometry. Spontaneous apoptosis was low (5%) in cALL and ALL-T and higher (8%) in NHL and cALL + My. A high frequency of bcl-2 expression was noted in cALL and cALL + My. A high frequency of p-gp expression was observed in cALL + My, ALL-T, and NHL. There was a reverse association between bcl-2 expression and spontaneous apoptosis. DXM-induced apoptosis was observed in 52.63%, TNF-induced in 42.85%, VCR-induced in 36.36%, and GM-CSF-induced in 33.3% of leukaemia and lymphoma cases. DXM and GM-CSF-driven apoptosis was reversibly associated with bcl-2-expression (bcl-2-dependent mechanism). VCR and TNF-driven apoptosis was not associated with bcl-2 expression, suggesting a different, bcl-2-independent, mechanism(s) of its induction. The in vitro induction of apoptosis was not associated with expression of p-gp. |
| Databáze: | OpenAIRE |
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