Virulence and antimicrobial susceptibility of clinical and environmental strains of Aeromonas spp. from northeastern Brazil
| Autor: | Célia Maria de Souza Sampaio, Raimunda Sâmia Nogueira Brilhante, David Caldas Vasconcelos, Giovanna Barbosa Riello, André Jalles Monteiro, Jamille Alencar Sales, Débora de Souza Collares Maia Castelo-Branco, Yago Brito de Ponte, Isis Sousa Bezerra de Menezes, Glaucia Morgana de Melo Guedes, José Júlio Costa Sidrim, José Luciano Bezerra Moreira, Marcos Fábio Gadelha Rocha, Manoel de Araújo Neto Paiva, Tereza de Jesus Pinheiro Gomes Bandeira, Lucas Pereira de Alencar, Rossana de Aguiar Cordeiro |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Imipenem
Aeromonas caviae Immunology Virulence Ceftazidime Microbial Sensitivity Tests Applied Microbiology and Biotechnology Microbiology Antibiotic resistance Drug Resistance Bacterial Genetics medicine Environmental Microbiology Humans Molecular Biology biology General Medicine biology.organism_classification Virology Aeromonas hydrophila Aeromonas Brazil medicine.drug Aeromonas veronii |
| Zdroj: | Canadian journal of microbiology. 61(8) |
| ISSN: | 1480-3275 |
| Popis: | The aims of the present study were to isolate and identify clinical and environmental strains of Aeromonas spp. by means of biochemical tests and the automated method VITEK 2 and to investigate the presence of the virulence genes cytotoxic enterotoxin (act), hemolysin (asa-1), and type III secretion system (ascV), and also the in vitro antimicrobial susceptibility of the strains. From the clinical isolates, 19 Aeromonas hydrophila, 3 Aeromonas veronii bv. sobria, and 1 Aeromonas caviae were identified, while from the environmental strains, 11 A. hydrophila, 22 A. veronii bv. sobria, 1 A. veronii bv. veronii, and 1 A. caviae were recovered. The gene act was detected in 69.5% of clinical isolates, asa-1 in 8.6%, and ascV in 34.7%. In the environmental strains, the detection rates were 51.4%, 45.7%, and 54.2% for the genes act, asa-1, and ascV, respectively. Resistance to amoxicillin–clavulanate and piperacillin–tazobactam was observed in 15 and 3 clinical strains, respectively, and resistance to ceftazidime, meropenem, imipenem, ciprofloxacin, and trimethoprim–sulfamethoxazole was observed in 1 strain for each drug. Resistance to amoxicillin–clavulanate and piperacillin–tazobactam was detected in 17 and 1 environmental strain, respectively. Higher resistance percentages were observed in clinical strains, but environmental strains also showed this phenomenon and presented a higher detection rate of virulence genes. Thus, it is important to monitor the antimicrobial susceptibility and pathogenic potential of the environmental isolates. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|