STEADY STATE MAINTENANCE OF ELECTROLYTES IN THE SPINAL CORD OF THE FROG
| Autor: | C. Paul Bianchi, S. D. Erulkar |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Sucrose
Sodium chemistry.chemical_element Electrolyte Zinc Calcium Biochemistry Chloride Electrolytes Cellular and Molecular Neuroscience Chlorides Caffeine medicine Animals Magnesium Rana pipiens Inulin Anatomy Spinal cord medicine.anatomical_structure Spinal Cord chemistry Steady state (chemistry) Anura Nuclear chemistry medicine.drug |
| Zdroj: | Journal of Neurochemistry. 32:1671-1677 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1979.tb02278.x |
| Popis: | Isolated frog spinal cords equilibrated from 3 to 24 h in Ringer's solution maintained steady state conditions with regard to electrolyte composition. Total sodium and chloride contents measured on the same spinal cords were found to be nearly equivalent (Na = 46.6 ± 1.4μmol/g wet wt vs Cl = 46.2 ± 1.2μmol/g wet wt). Calcium and magnesium contents were 3.0 ± 0.5 and 4.8 ± 0.2pμol/g wet wt respectively for fresh spinal cords and 2.1 ± 0.4 and 5.5 ± 0.9pmol/g wet wt respectively for spinal cords equilibrated for 24 h. Zinc content was 0.29 ± 0.01 μmol/g wet wt. Insulin space was found to be smaller than sucrose space (0.24 ml/g vs 0.37 ml/g). Sodium and chloride spaces were slightly higher than sucrose space. Sodium and chloride in the non-sucrose space was 4.8 and 9.5 μmol/g wet wt. Residual radioactive sodium, or sodium content of spinal cords washed out for 180 min in non-radioactive Ringer's solution or in choline or lithium Ringer's solution, was 4.2 ± 0.4μmol/g wet wt (n = 9). The agreement between residual sodium content and sodium in the non-sucrose space suggests that the mean intracellular sodium content of the spinal cord neurons is low. |
| Databáze: | OpenAIRE |
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