A sephadex column radioimmunoassay of unconjugated estriol in pregnancy plasma

Autor: Marion C. Fetter, James Edward Christner
Rok vydání: 1974
Předmět:
Zdroj: Steroids. 24(3)
ISSN: 0039-128X
Popis: A rapid, precise, and accurate radioimmunoassay for unconjugated estriol in pregnancy plasma has been developed which makes use of the adsorptive properties of Sephadex. The estriol is extracted from plasma by adsorption onto a small column of Sephadex. After the proteins and other interfering materials are washed away, the estriol is equilibrated with a limiting amount of specific antibody. The Sephadex column serves as a means of separating the bound from the unbound estriol, the ratio of which is determined by adding to the system tracer amounts of tritium labeled hormone. The sensitivity is about 220 pg in the sample. The intra- and inter-assay coefficient of variation is 5–6%. The method correlated well with one which involved purification of the estriol prior to quantitationby radioiommunoassay.
Databáze: OpenAIRE