A sephadex column radioimmunoassay of unconjugated estriol in pregnancy plasma
Autor: | Marion C. Fetter, James Edward Christner |
---|---|
Rok vydání: | 1974 |
Předmět: |
Time Factors
Coefficient of variation Clinical Biochemistry Radioimmunoassay Cross Reactions Tritium Biochemistry Structure-Activity Relationship Endocrinology Unconjugated estriol Pregnancy Blood plasma Animals Humans Molecular Biology reproductive and urinary physiology Pharmacology Sephadex column Analysis of Variance Chromatography Chemistry Estriol Microchemistry Organic Chemistry Blood Proteins Sephadex Evaluation Studies as Topic Chromatography Gel Female Chromatography Thin Layer Rabbits hormones hormone substitutes and hormone antagonists Protein Binding |
Zdroj: | Steroids. 24(3) |
ISSN: | 0039-128X |
Popis: | A rapid, precise, and accurate radioimmunoassay for unconjugated estriol in pregnancy plasma has been developed which makes use of the adsorptive properties of Sephadex. The estriol is extracted from plasma by adsorption onto a small column of Sephadex. After the proteins and other interfering materials are washed away, the estriol is equilibrated with a limiting amount of specific antibody. The Sephadex column serves as a means of separating the bound from the unbound estriol, the ratio of which is determined by adding to the system tracer amounts of tritium labeled hormone. The sensitivity is about 220 pg in the sample. The intra- and inter-assay coefficient of variation is 5–6%. The method correlated well with one which involved purification of the estriol prior to quantitationby radioiommunoassay. |
Databáze: | OpenAIRE |
Externí odkaz: |