Ultrasonic alignment of bio-functionalized magnetic beads and live cells in PDMS micro-fluidic channel
| Autor: | Seung Hyun Cho, T.S. Ramulu, CheolGi Kim, Afroja Tazin Islam, Young-Jae Eu, Venu Reddy, A. K. M. Ariful Haque Siddique |
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| Rok vydání: | 2012 |
| Předmět: |
Materials science
Channel (digital image) Cell Survival Biomedical Engineering Analytical chemistry Standing wave chemistry.chemical_compound Ultrasonics Dimethylpolysiloxanes Particle Size Molecular Biology business.industry Ultrasound Synechocystis Resonance Equipment Design Microfluidic Analytical Techniques Sample (graphics) Microspheres Nylons Transducer Magnetic Fields chemistry Optoelectronics Polystyrenes Ultrasonic sensor Polystyrene business |
| Zdroj: | Biomedical microdevices. 14(6) |
| ISSN: | 1572-8781 |
| Popis: | In this work, we demonstrated the alignment of polystyrene latex microspheres (diameter of 1 ~45 μm), bio-functionalized superparamagnetic beads (diameter 2.8 μm), and live cells (average diameter 1 ~2 μm) using an ultrasonic standing wave (USW) in a PDMS microfluidic channel (330 μm width) attached on a Si substrate for bio-medical applications. To generate a standing wave inside the channel, ultrasound of 2.25 MHz resonance frequency (for the channel width) was applied by two ultrasound transducers installed at both sides of the channel which caused the radiation force to concentrate the micro-particles at the single pressure nodal plane of USW. By increasing the frequency to the next resonance condition of the channel, the particles were concentrated in dual nodal planes. Migration time of the micro-particles towards the single nodal plane was recorded as 108 s, 17 s, and 115 s for polystyrene particles of 2 μm diameter, bio-functionalized magnetic beads, and live cells, respectively. These successful alignments of the bio-functionalized magnetic beads along the desired part of the channel can enhance the performance of a sensor which is applicable for the bio-hybrid system and the alignment of live cells without any damage can be used for sample pre-treatment for the application of lab-on-a-chip type bioassays. |
| Databáze: | OpenAIRE |
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