Cloning and Expression of the Chick Anti-Müllerian Hormone Gene
| Autor: | Rodolfo Rey, Claude Pieau, Nathalie di Clemente, Danièle Carré Eusèbe, Jean-Yves Picard, B. Vigier, Nathalie Josso |
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| Rok vydání: | 1996 |
| Předmět: |
Anti-Mullerian Hormone
Male DNA Complementary animal structures Transcription Genetic Mullerian Ducts Molecular Sequence Data Gene Expression Chick Embryo Biology Mullerian duct regression Polymerase Chain Reaction Biochemistry Mice Organ Culture Techniques Complementary DNA Testis medicine Animals Humans Amino Acid Sequence Cloning Molecular Molecular Biology Gene Conserved Sequence DNA Primers Glycoproteins Mammals Sexual differentiation Base Sequence Sequence Homology Amino Acid Embryo Cell Biology Blotting Northern Sertoli cell Molecular biology Growth Inhibitors Introns Rats Testicular Hormones medicine.anatomical_structure Expression cloning Cattle Chickens |
| Zdroj: | Journal of Biological Chemistry. 271:4798-4804 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.271.9.4798 |
| Popis: | Müllerian duct regression in male embryos is due to early production by fetal Sertoli cells of anti-Müllerian hormone, a homodimeric protein of the transforming growth factor- beta superfamily. In mammals, both female Müllerian ducts develop into the uterus and Fallopian tubes, whereas in birds, the right oviduct does not develop. To gain insight into sex differentiation in birds, we have cloned the cDNA for chick anti-Müllerian hormone using antibodies raised against the partially purified protein. Expression cloning was required because of the lack of cross-hybridization between mammalian and chick anti-Müllerian hormone DNA. The chick DNA and protein are significantly longer, due to insertions that abolish nucleotide homology, except in the cDNA coding for the C-terminal, bioactive part of the protein. Nevertheless, the general structure of the gene, sequenced from the transcription initiation to the polyadenylation site, and the main features of the protein are conserved between the chick and mammals. The chick anti-Müllerian hormone gene is expressed at high levels in Sertoli cells of the embryonic testes and in lower amounts in both ovaries, higher levels being reached on the left side after 10 days of incubation. |
| Databáze: | OpenAIRE |
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