The Influence of the Degradation of Tetracycline by Free Radicals from Riboflavin-5′-Phosphate Photolysis on Microbial Viability
| Autor: | Chun-Yi Wu, Jeu-Ming P. Yuann, Shiuh-Tsuen Huang, Chien-Wei Cheng, Song-Hua Wang, Shwu-Yuan Lee, Ji-Yuan Liang, Sin He |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030103 biophysics animal structures Tetracycline Radical Flavin mononucleotide Riboflavin free radicals 02 engineering and technology medicine.disease_cause Microbiology Article 03 medical and health sciences chemistry.chemical_compound Virology medicine heterocyclic compounds Irradiation riboflavin-5′-phosphate lcsh:QH301-705.5 Escherichia coli tetracycline digestive oral and skin physiology Photodissociation food and beverages 021001 nanoscience & nanotechnology Phosphate blue light lcsh:Biology (General) chemistry photolysis 0210 nano-technology Nuclear chemistry medicine.drug |
| Zdroj: | Microorganisms Microorganisms, Vol 7, Iss 11, p 500 (2019) Volume 7 Issue 11 |
| ISSN: | 2076-2607 |
| Popis: | Tetracycline (TC) is a broad-spectrum antibiotic compound. Wastewater with TC may have an adverse effect on ecosystems. Riboflavin-5&prime phosphate (FMN or flavin mononucleotide) is a non-toxic product of the phosphorylation of vitamin B2 and is required for the proper functioning of the humans. FMN is sensitized to ultraviolet (UV) and blue light radiation, as evidenced by the generation of reactive oxygen species (ROS). This study inspects feasible applications of blue light on FMN so as to develop a valid way of degrading TC by FMN photolysis. We used the increased rate of bacterial survival as a practical indicator of antibiotic degradation. TC in the presence of FMN solution decomposed completely after 20 W/m2 of blue light irradiation (TCF treatment), and the degradation of TC (D-TCF) occurred after the photolytic process. After TCF treatment, colony-forming units (CFUs) of Escherichia coli (E. coli) were determined for the D-TCF solution. The CFU of E. coli preservation was 93.2% of the D-TCF solution (50 &mu g/mL of TC in the presence of 114 &mu g/mL of FMN solution treated with 20 W/m2 of blue light irradiation at 25 ° C for 1 h) cultivation. The mass spectrum of D-TCF showed diagnostic ion signals at m/z 431.0 and 414.0 Da. The molecular formula of D-TCF was C21H22N2O8, and the exact mass was 430.44 g/mol. TC degradation by FMN photolysis can significantly decrease the antimicrobial ability of TC. The results expressed here regarding the influence of FMN photolysis on TC degradation offer an environmentally sound wastewater treatment method. |
| Databáze: | OpenAIRE |
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