In Ovo Administration of Silver Nanoparticles and/or Amino Acids Influence Metabolism and Immune Gene Expression in Chicken Embryos

Autor: Natalia Kurantowicz, André Chwalibog, Krishna Prasad Vadalasetty, Lane Pineda, Anna Hotowy, Manish Mehra, Ewa Sawosz, Subrat K Bhanja
Rok vydání: 2015
Předmět:
Lipopolysaccharides
Threonine
chicken embryo
Lipopolysaccharide
medicine.medical_treatment
Metal Nanoparticles
Chick Embryo
lcsh:Chemistry
chemistry.chemical_compound
Gene expression
Nanotechnology
Insulin-Like Growth Factor I
lcsh:QH301-705.5
Spectroscopy
chemistry.chemical_classification
Gene Expression Regulation
Developmental
General Medicine
immune gene expression
Computer Science Applications
Amino acid
Interleukin-10
Biochemistry
embryonic structures
silver nanoparticles
animal structures
Silver
Biology
In ovo
Catalysis
Article
Inorganic Chemistry
Oxygen Consumption
medicine
Animals
Colloids
Cysteine
Physical and Theoretical Chemistry
Molecular Biology
Tumor Necrosis Factor-alpha
Growth factor
Gene Expression Profiling
Organic Chemistry
Metabolism
Molecular biology
Toll-Like Receptor 2
Toll-Like Receptor 4
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Immune System
metabolism
Spleen
Zdroj: International Journal of Molecular Sciences
Bhanja, S K, Hotowy, A M, Mehra, M, Sawosz, E, Pineda, L M, Vadalasetty, R K P, Kurantowicz, N & Chwalibog, A 2015, ' In Ovo administration of silver nanoparticles and/or amino acids influence metabolism and immune gene expression in chicken embryos ', International Journal of Molecular Sciences (Online), vol. 16, no. 5, pp. 9484-9503 . https://doi.org/10.3390/ijms16059484
International Journal of Molecular Sciences, Vol 16, Iss 5, Pp 9484-9503 (2015)
Volume 16
Issue 5
Pages 9484-9503
ISSN: 1422-0067
DOI: 10.3390/ijms16059484
Popis: Due to their physicochemical and biological properties, silver nanoparticles (NanoAg) have a wide range of applications. In the present study, their roles as a carrier of nutrients and an immunomodulator were tested in chicken embryos. Cysteine (Cys)+NanoAg injected embryos had smaller livers but heavier breasts on the 19th day of embryogenesis. Cys injected embryos had lower oxygen consumption compared to threonine (Thr) or NanoAg injected embryos. The energy expenditure in Thr+NanoAg, or NanoAg injected embryos was higher than Cys or Cys+NanoAg but was not different from uninjected control embryos. Relative expression of the hepatic insulin-like growth factor-I (IGF-I) gene was higher in Cys or NanoAg injected embryos after lipopolysaccharide (LPS) induction. The gene expression of hepatic tumour necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) did not differ among amino acids, NanoAg and uninjected controls in the non-LPS groups, but increased by many folds in the LPS treated NanoAg, Cys and Cys+NanoAg groups. In LPS treated spleens, TNF-α expression was also up-regulated by NanoAg, amino acids and their combinations, but interleukin-10 (IL-10) expression was down-regulated in Thr, Cys or Thr+NanoAg injected embryos. Toll like receptor-2 (TLR2) expression did not differ in NanoAg or amino acids injected embryos
however, toll like receptor-4 (TLR4) expression was higher in all treated embryos, except for Cys+NanoAg, than in uninjected control embryos. We concluded that NanoAg either alone or in combination with amino acids did not affect embryonic growth but improved immunocompetence, indicating that NanoAg and amino acid complexes can act as potential agents for the enhancement of innate and adaptive immunity in chicken.
Databáze: OpenAIRE
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