Low prevalence of transcriptionally active human papilloma virus in Indian patients with HNSCC and leukoplakia

Autor: Shubhada Kane, Manishkumar Pandey, Asawari Patil, Kumar Prabhash, Rajiv S. Desai, Priyanka G. Bhosale, Manoj B. Mahimkar
Rok vydání: 2015
Předmět:
0301 basic medicine
Male
Saliva
Pathology
Polymerase Chain Reaction
law.invention
Human Papillomavirus DNA Tests
0302 clinical medicine
law
Human Papillomavirus DNA Test
Polymerase chain reaction
In Situ Hybridization
Leukoplakia
Human papillomavirus 16
virus diseases
Middle Aged
Immunohistochemistry
female genital diseases and pregnancy complications
Head and Neck Neoplasms
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
Female
Oral Surgery
Adult
medicine.medical_specialty
India
Virus
Pathology and Forensic Medicine
03 medical and health sciences
stomatognathic system
otorhinolaryngologic diseases
medicine
Biomarkers
Tumor
Humans
Radiology
Nuclear Medicine and imaging
Dentistry (miscellaneous)
business.industry
Squamous Cell Carcinoma of Head and Neck
Papillomavirus Infections
medicine.disease
Head and neck squamous-cell carcinoma
stomatognathic diseases
030104 developmental biology
DNA
Viral
Surgery
business
Nested polymerase chain reaction
Zdroj: Oral surgery, oral medicine, oral pathology and oral radiology. 122(5)
ISSN: 2212-4411
Popis: Objectives In the present study, we comprehensively analyzed the prevalence of transcriptionally active human papilloma virus (HPV) in tissue samples of Indian patients with leukoplakia, predominantly hyperplastic lesions and head and neck squamous cell carcinoma (HNSCC). In addition, saliva samples from patients with HNSCC were screened for HPV detection. Study Design P16 overexpression was analyzed by immunohistochemistry. Tissue samples of leukoplakia (n = 121) and HNSCC (n = 427) and saliva from patients with HNSCC (n = 215) were tested for HPV using nested polymerase chain reaction. Positive samples were sequenced for subtyping. The presence of HPV E6/E7 mRNA was confirmed by RNA in situ hybridization. Results P16 expression and HPV DNA were not detected in any of the leukoplakia specimens. Of the 427 HNSCC tumors, 9 showed p16 overexpression and 7/427 cases were positive for HPV16 DNA, in saliva or tissue. E6/E7 mRNA positivity was observed in 8 HNSCC samples, primarily from patients with no habit of tobacco consumption. The prevalence of high-risk HPV was restricted to oropharynx and larynx, with very little concordance between p16 overexpression and HPV positivity. All patients with HPV-positive saliva samples had transcriptionally active HPV present in their tumors. Conclusion The presence of HPV DNA does not necessarily reflect transcriptionally active virus in tumors; hence, it is important to consider this fact while categorizing HPV-associated tumors.
Databáze: OpenAIRE
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