Physicochemical behaviour and biological activity of bovine growth hormone in acidic solution
| Autor: | María Amelia Enero, J. M. Dellacha, Alejandro C. Paladini |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Chemical Phenomena
Size-exclusion chromatography Salt (chemistry) Hydrochloric acid Buffers Biochemistry Genetics and Molecular Biology (miscellaneous) chemistry.chemical_compound Drug Stability Animals Bovine somatotropin chemistry.chemical_classification Chromatography Viscosity Elution Biological activity Hydrogen-Ion Concentration Electrophoresis Disc Molecular Weight Solutions Solvent Chemistry chemistry Growth Hormone Glycine Chromatography Gel Biological Assay Cattle Dialysis Ultracentrifugation |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure. 168:95-105 |
| ISSN: | 0005-2795 |
| DOI: | 10.1016/0005-2795(68)90238-9 |
| Popis: | 1. 1.|The variation of some physicochemical and biological properties of bovine growth hormone dissolved in various acidic buffers, has been examined. 2. 2.|The hormone preparation used was not contaminated with acid proteases. 3. 3.|Measurements of sedimentation velocities, intrinsic viscosities, half-escape times in analytical dialysis and elution volumes in gel filtration with hormone solutions in glycine-hydrochloric acid buffers, indicate conformation changes of the molecule between pH values 2.2 and 3.6. These changes do not affect the growth-promoting activity of the hormone. 4. 4.|The addition of neutral salts to the acidic glycine buffers promotes an association whose extension is dependent on the concentration of salt and the nature of the anion. The associated hormone has decreased biological activity. 5. 5.|The maximum physicochemical and biological stability of the hormone in acidic solution was found in glycine hydrochloric acid buffer, I 0.010, at pH 3.6. The s201% obtained in this solvent is 2.3 and corresponds to a molecular weight in the neighbourhood at 21 000. |
| Databáze: | OpenAIRE |
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