Estrogen Receptor α Is a Novel Marker Expressed by Follicular Dendritic Cells in Lymph Nodes and Tumor-Associated Lymphoid Infiltrates

Autor: Roberto Chiarle, Anna Sapino, Enza Ferrero, Gianni Bussolati, Paola Cassoni, Pellegrino Crafa, Massimo Bongiovanni, Nicoletta Fortunati, Luisella Righi
Rok vydání: 2003
Předmět:
Adult
Male
Pathology
medicine.medical_specialty
CARCINOMA
HUMAN BREAST-CANCER
ANTIGEN
Estrogen receptor
Biology
Immunophenotyping
Pathology and Forensic Medicine
RETICULUM CELLS
Lymphocytes
Tumor-Infiltrating
NONSTEROIDAL ANTIESTROGENS INHIBIT
medicine
Humans
CD21
Tissue Distribution
RNA
Messenger
HUMAN BREAST-CANCER
VARIANT MESSENGER-RNAS
NONSTEROIDAL ANTIESTROGENS INHIBIT
HODGKINS-DISEASE
T-CELLS
RETICULUM CELLS
CARCINOMA
ANTIGEN
CD21
DIFFERENTIATION
Lymph node
Aged
HODGKINS-DISEASE
Hyperplasia
Follicular dendritic cells
Reverse Transcriptase Polymerase Chain Reaction
Estrogen Antagonists
Estrogen Receptor alpha
CD23
Germinal center
Dendritic cell
Middle Aged
Immunohistochemistry
Androgen receptor
Tamoxifen
DIFFERENTIATION
medicine.anatomical_structure
Receptors
Estrogen
Lymphatic Metastasis
T-CELLS
Female
VARIANT MESSENGER-RNAS
Lymph Nodes
Lymph
Receptors
Progesterone
Biomarkers
Dendritic Cells
Follicular
Regular Articles
Zdroj: Scopus-Elsevier
ISSN: 0002-9440
DOI: 10.1016/s0002-9440(10)63490-6
Popis: During routine assessment of the hormonal phenotype of breast carcinomas, we detected expression of the estrogen receptor (ER) in the germinal centers of reactive lymphoid follicles surrounding malignant foci. To confirm and extend this finding, we compared ER-alpha, progesterone receptor (PR), and androgen receptor (AR) immunostaining in hyperplastic or metastatic lymph nodes obtained from patients with various pathology, disease location, gender and age. Irrespective of these parameters, we found that: 1) ER-alpha-positive cells were located prevalently in germinal centers, 2) the PR was weakly expressed by cells within and surrounding germinal centers, and 3) the androgen receptor was undetectable. Transcripts for ER-alpha and PR were also detected by reverse transcription-polymerase chain reaction on laser-microdissected lymph node germinal centers. Morphologically, the ER-positive cells resemble dendritic cells and by double immunostaining were found to express both CD21 and CD23, which is characteristic of follicular dendritic cells. Finally, we assessed the effects of Tamoxifen treatment by comparing the numbers of ER-positive follicular dendritic cells in lymph nodes obtained from breast cancer patients before and after treatment. The results show that Tamoxifen treatment generated larger germinal centers with more abundant ER(+)/CD21(+)/CD23(+) cells. Taken together, these results open new perspectives on the effects of sex steroids and their antagonists on the human response in cancer and inflammation.
Databáze: OpenAIRE
Externí odkaz: