Colonization of Rhodnius prolixus gut by Trypanosoma cruzi involves an extensive parasite killing
| Autor: | Luciana de Lima Ferreira, Juliana Alves-Silva, Christian Probst, Alessandra A. Guarneri, Rafaela M.M. Paim, Rafael Luis Kessler, Roberta Carvalho Ferreira, Marcelo G. Lorenzo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chagas disease Nymph Trypanosoma cruzi 030231 tropical medicine Rhodnius Real-Time Polymerase Chain Reaction Parasite load Microbiology 03 medical and health sciences Mice 0302 clinical medicine parasitic diseases medicine Parasite hosting Animals Chagas Disease Rhodnius prolixus Incubation Analysis of Variance biology Midgut DNA Protozoan biology.organism_classification medicine.disease Insect Vectors 030104 developmental biology Infectious Diseases Immunology Animal Science and Zoology Parasitology |
| Zdroj: | Parasitology. 143(4) |
| ISSN: | 1469-8161 |
| Popis: | SUMMARYTrypanosoma cruzi, the etiological agent of Chagas disease, is ingested by triatomines during their bloodmeal on an infected mammal. Aiming to investigate the development and differentiation of T. cruzi inside the intestinal tract of Rhodnius prolixus at the beginning of infection we fed insects with cultured epimastigotes and blood trypomastigotes from infected mice to determine the amount of recovered parasites after ingestion. Approximately 20% of the ingested parasites was found in the insect anterior midgut (AM) 3 h after feeding. Interestingly, a significant reduction (80%) in the numbers of trypomastigotes was observed after 24 h of infection suggesting that parasites were killed in the AM. Moreover, few parasites were found in that intestinal portion after 96 h of infection. The evaluation of the numbers of parasites in the posterior midgut (PM) at the same periods showed a reduced parasite load, indicating that parasites were not moving from the AM. Additionally, incubation of blood trypomastigotes with extracts from R. prolixus AMs revealed that components of this tissue could induce significant death of T. cruzi. Finally, we observed that differentiation from trypomastigotes to epimastigotes is not completed in the AM; instead we suggest that trypomastigotes change to intermediary forms before their migration to the PM, where differentiation to epimastigotes takes place. The present work clarifies controversial points concerning T. cruzi development in insect vector, showing that parasite suffers a drastic decrease in population size before epimastigonesis accomplishment in PM. |
| Databáze: | OpenAIRE |
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