Plasmodium: immunization with carboxyl-terminal regions of MSP-1 protects against homologous but not heterologous blood-stage parasite challenge
| Autor: | Thomas M. Daly, Harris L. Rotman, Carole A. Long |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Plasmodium Plasmodium berghei Recombinant Fusion Proteins Immunology Molecular Sequence Data Heterologous Antibodies Protozoan Parasitemia Plasmodium chabaudi Mice Species Specificity Antibody Specificity parasitic diseases medicine Parasite hosting Animals Amino Acid Sequence Merozoite Surface Protein 1 Mice Inbred BALB C biology General Medicine Plasmodium yoelii medicine.disease biology.organism_classification Fusion protein Virology Malaria Infectious Diseases biology.protein Parasitology Immunization Antibody |
| Zdroj: | Experimental parasitology. 91(1) |
| ISSN: | 0014-4894 |
| Popis: | Rotman, H. L., Daly, T. M., and Long, C. A. 1999.Plasmodium: Immunization with carboxyl-terminal regions of MSP-1 protects against homologous but not heterologous blood-stage parasite challenge.Experimental Parasitology91, 78–85. A leading candidate for a vaccine targeted at the erythrocytic stages of plasmodial parasite development is the merozoite surface protein-1 (MSP-1). We have previously shown that the carboxyl-terminal region of MSP-1 derived fromPlasmodium yoelii yoelii17XL, expressed as a fusion protein with glutathioneS-transferase (GST-PYC2), can immunize mice against an otherwise lethal homologous challenge infection. This protection has been shown to be predominantly mediated by antibodies. We report here on the efficacy of immunization with MSP-1 carboxyl regions when the challenge is a heterologous rodent parasite species. The course of parasitemia was not altered in mice immunized with GST-PYC2 and challenged with 104heterologousPlasmodium chabaudi adamiparasites, as both control and immunized mice developed infections that peaked at day 7 and then rapidly declined. Similarly, mice immunized with GST-PYC2 and challenged with 105Plasmodium bergheiANKA parasites displayed virulence similar to that seen in infection control mice.The homologous region of theP. chabaudi adamiMSP-1 gene was similarly expressed as a fusion protein with GST. Mice immunized with GST-PCC2 and challenged with 104parasites showed significant protection against homologousP. chabaudi adamiinfection but no protection whatsoever against heterologousP. yoelii yoelii17XL infection.Thesein vivoresults correlate with the observation that sera generated by immunization with the carboxyl region of MSP-1 recognizes this protein from homologous, but not heterologous, radiolabeled parasite protein preparations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect