Modification of in vitro immune lymphocyte-target cell interaction by some biologically active drugs
| Autor: | Boris D Brondz, Antonina E Snegiröva, Olga G Shamborant, Yurij A Rassulin |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Time Factors
Cell Survival Prednisolone Lymphocyte Neuraminidase Ultrafiltration Mice Inbred Strains Physical Therapy Sports Therapy and Rehabilitation Pronase Mice chemistry.chemical_compound Immune system medicine Animals Cytotoxic T cell Trypsin Lymphocytes Staining and Labeling biology Heparin Macrophages Polysaccharides Bacterial Rehabilitation Immunity Chloroquine Sarcoma Trypan Blue General Medicine Chromatography Ion Exchange Cytotoxicity Tests Immunologic Molecular biology In vitro Mice Inbred C57BL Kinetics medicine.anatomical_structure chemistry Phospholipases biology.protein Spectrophotometry Ultraviolet Trypan blue Rabbits medicine.drug |
| Zdroj: | Immunochemistry. 10:175-189 |
| ISSN: | 0019-2791 |
| Popis: | The conditions and mechanisms of the action of a number of drugs on the non-cytotoxic concentrations on the in vitro cytotoxic effect (CE)∗ of immune lymphocytes have been studied. Pronase and heparin appeared to be the most powerful inhibitors of CE, phospholipase C was ineffective, neuraminidase amplified CE when target cells were pretreated. The initial step of immune lymphocyte interaction with target cells completed by the specific lymphocyte absorption was inhibited entirely by pronase, partially by trypsin and Vi-polysaccharide, was not inhibited by heparin, trypan blue, chlorochin and prednisolon and was enhanced by neuraminidase. The pronase action was completely reversible. Heparin affected only overall byt not specific lymphocyte-target contact and suppressed blast percentage growth subsequent to this contact. The conclusive step of interaction, target cell destruction, has been inhibited by pronase and lysosome-active drugs (trypan blue, chlorochin, prednisolon). The latter did not affect either overall or specific lymphocyte absorption or an increase of blast percentage during incubation with appropriate target cells. |
| Databáze: | OpenAIRE |
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