Macro- and Microstructure of Erythrocyte Membranes Under Acute Massive Hemorrhage and Subsequent Blood Reinfusion
| Autor: | V.V. Alexandrin, Viktor Moroz, I.S. Novodergkina, A. M. Chernysh, A.K. Kirsanova, Elena Kozlova |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Resuscitation medicine.medical_specialty Hemorrhage Microscopy Atomic Force Animals Medicine Blood Transfusion Acute hemorrhage Fourier Analysis business.industry Atomic force microscopy Erythrocyte Membrane Hemorrhagic hypotension Rodent model Microstructure Rats Surgery Erythrocyte membrane Anesthesiology and Pain Medicine Membrane Reperfusion Biophysics Hypotension Cardiology and Cardiovascular Medicine business |
| Zdroj: | Seminars in Cardiothoracic and Vascular Anesthesia. 14:248-255 |
| ISSN: | 1940-5596 1089-2532 |
| DOI: | 10.1177/1089253210388518 |
| Popis: | The authors studied changes in erythrocyte membrane nanostructure using a rodent model of hemorrhagic hypotension and resuscitation. Both macro- and microstructural elements were examined using atomic force microscopy. Membrane “roughness” was characterized using spatial Fourier transformation and was stratified according to the periodicity of the membrane. Acute hemorrhage resulted in an increase in the diameter and height of erythrocytes, which returned to baseline levels by the end of the hemorrhagic hypotensive period. The effect of hypotension on the erythrocyte surface was nonuniform. In those regions where damage was considerable, the rate of restoration of the membrane microstructure to baseline levels was prolonged. The less damaged surfaces were restored more rapidly to control values after reperfusion. More detailed use of atomic force microscopy in the definition of the erythrocyte membrane microstructure may further define the mechanisms of cellular functional restoration after hemorrhage. |
| Databáze: | OpenAIRE |
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